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Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin

ABSTRACT: Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin

PROVIDER: PRJNA254080 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1503350.fastq.gz	Fastqsanger.gz
	SRR1503351.fastq.gz	Fastqsanger.gz
	SRR1503346.fastq.gz	Fastqsanger.gz
	SRR1503347.fastq.gz	Fastqsanger.gz
	SRR1503348.fastq.gz	Fastqsanger.gz

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Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [ChIP-seq]

Project description:Meiotic recombination hotspots are associated with histone post-translational modifications and open chromatin. However, it remains unclear how histone modifications and chromatin structure directly regulate meiotic recombination. Here, we identify acetylation of histone H4 at Lys44 (H4K44ac) as a new histone modification, occurring on the nucleosomal lateral surface. We show that H4K44ac is specific to yeast sporulation, rising during yeast meiosis and displaying genome-wide enrichment at recombination hotspots in meiosis. The H4K44 residue is required for normal meiotic recombination, for normal levels of double strand breaks during meiosis, and for optimal sporulation. Non-modifiable substitution H4K44R results in reduced MNase digestion and decreased binding of recombination-associated proteins at hotspots. Our results show that H4K44ac creates an accessible chromatin environment for key proteins to facilitate meiotic recombination. One sample, H4K44ac chIP from 4hrs sporulation yeast and one background H4 chIP from the same, two replicates each Two replicates each of H3K4me3 and H3K56ac chIP-seq in WT and H4K44->R mutant yeast, with two replicates of H3 chIP-seq in each genetic background Two replicates each of Rad51 chIP-seq in WT and H4K44->R mutant yeast with a single replicate of accompanying input DNA in each genetic background

2015-12-07 | E-GEOD-59003 | biostudies-arrayexpress

Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [Mnase-seq]

Project description:Meiotic recombination hotspots are associated with histone post-translational modifications and open chromatin. However, it remains unclear how histone modifications and chromatin structure directly regulate meiotic recombination. Here, we identify acetylation of histone H4 at Lys44 (H4K44ac) as a new histone modification, occurring on the nucleosomal lateral surface. We show that H4K44ac is specific to yeast sporulation, rising during yeast meiosis and displaying genome-wide enrichment at recombination hotspots in meiosis. The H4K44 residue is required for normal meiotic recombination, for normal levels of double strand breaks during meiosis, and for optimal sporulation. Non-modifiable substitution H4K44R results in reduced MNase digestion and decreased binding of recombination-associated proteins at hotspots. Our results show that H4K44ac creates an accessible chromatin environment for key proteins to facilitate meiotic recombination. Two samples, one WT MNase-seq and one MNase-seq from yeast with a lysine->arginine mutation at H4K44, no replicates Two replicates each of MNase-seq in WT and H4K44->R mutant yeast grown in YPD or YPA.

2015-12-07 | E-GEOD-59004 | biostudies-arrayexpress

Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [Mnase-seq]

2015-12-07 | GSE59004 | GEO

Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [ChIP-seq]

2015-12-07 | GSE59003 | GEO

Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-12-07 | E-GEOD-59005 | biostudies-arrayexpress

Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-12-07 | GSE59005 | GEO

Saccharomyces cerevisiae

Project description:Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [ChIP-seq]

| PRJNA254082 | ENA

Saccharomyces cerevisiae

Project description:Acetylation of histone H4 at lysine 44 facilitates meiotic recombination by creating accessible chromatin [Mnase-seq]

| PRJNA254081 | ENA

Glutarylation of Histone H4 Lysine 91 Regulates Chromatin Dynamics

Project description:Chromatin is decorated with diverse histone posttranslational modifications (PTMs) that are involved in regulating chromatin structure and dynamics during various DNA-associated processes such as gene transcription, DNA replication and DNA damage repair. Here, we combine a chemical reporter with mass spectrometry to identify a new class of histone PTM, lysine glutarylation (Kglu), occurring at 27 lysine residues on human core histones. The characterization of an evolutionarily conserved Kglu mark at histone H4 lysine 91 (H4K91glu) using a semisynthetic glutarylated protein reveals that this negatively charged modification greatly destabilizes nucleosome by weakening the interactions between histone H2A/H2B dimers and H3/H4 tetramer in vitro. The replacement of H4K91 by glutamic acid in S. cerevisiae to mimic glutarylation influences chromatin structure and thereby results in a global up-regulation of transcription and defects in cell cycle progression, DNA damage repair and telomere silencing in vivo. In mammalian cells, H4K91glu is mainly enriched at promoter regions of highly expressed genes. A down-regulation of H4K91glu is tightly associated with chromatin condensation during mitosis and in response to DNA damage. The cellular dynamics of H4K91glu is controlled by Sirt7 as a deglutarylase and KAT2A as a glutaryltransferase. This study designates a new histone mark (Kglu) as a new regulatory mechanism for chromatin dynamics.

2019-09-16 | GSE131807 | GEO

RNA-seq analysis of NHR-23-depleted, SPE-44-depleted, and NHR-23+SPE-44-depleted C. elegans

Project description:We performed RNA-seq analysis of control, NHR-23-depleted, SPE-44-depleted and NHR-23+SPE-44 depleted adult male animals, to identify genes regulated by NHR-23 and SPE-44.

2022-06-22 | GSE206676 | GEO

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