Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA255108 | ENA |
REPOSITORIES: ENA
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Project description:Exposures to environmental endocrine disruptors is growing and human contact in industrialized countries has become signficant and constant. We studied the effect of chronic exposure to two endocrine disrupting compound, bisphenol A and genistein, in an in vitro cell culture system. MCF7 cells were cultured for greater than 70 passages under normal (MCF7-F) conditions or with the addition of 50 nM BPA (MCF7-B) or GEN (MCF7-G). We performed transcriptome analysis of the all three cell lines in the absence of any estrogenic compounds and in the presence of 10 nM estradiol for 3 hours.
2014-09-01 | GSE59345 | GEO
Project description:MCF7 cells were exposed to xenoestrogens (vehicle = 0.1% ethanol, 30 pM 17beta-estradiol, 3 micromolar genistein, 10 micromolar genistein, 1 micomolar daidzein, 10 nM bisphenol A, 10 nM para-nonylphenol, 3 nM PPT) for 48 hours. Keywords: xenoestrogen response
2006-07-01 | GSE5200 | GEO
Project description:MCF7 cells were exposed to xenoestrogens (vehicle = 0.1% ethanol, 30 pM 17beta-estradiol, 3 micromolar genistein, 10 micromolar genistein, 1 micomolar daidzein, 10 nM bisphenol A, 10 nM para-nonylphenol, 3 nM PPT) for 48 hours. Experiment Overall Design: MCF7/BUS cells were exposed to xenoestrogens for 48 hours. Experiments were repeated three times independently.
2008-06-13 | E-GEOD-5200 | biostudies-arrayexpress
Project description:Exposures to environmental endocrine disruptors is growing and human contact in industrialized countries has become signficant and constant. We studied the effect of chronic exposure to two endocrine disrupting compound, bisphenol A and genistein, in an in vitro cell culture system. MCF7 cells were cultured for greater than 70 passages under normal (MCF7-F) conditions or with the addition of 50 nM BPA (MCF7-B) or GEN (MCF7-G). We performed transcriptome analysis of the all three cell lines in the absence of any estrogenic compounds and in the presence of 10 nM estradiol for 3 hours. The MCF7-F, B, G cell lines were starved of all estrogenic compounds by culturing the cells for 72 hours in phenol red free DMEM containing 5% charcoal/dextran treated FBS. Cells were then treated with either ethanol vehicle or 10 nM 17b-estradiol for 3 hours. Total RNA was harvested and utilized for whole genome analysis on the Affymetrix Human Trascriptome Array 2.0. The experiment was performed in duplicate
2014-09-01 | E-GEOD-59345 | biostudies-arrayexpress
Project description:Effects of plasticizers (bisphenol A, bisphenol AF) and an herbicide in MCF7 human breast cancer cells
| PRJNA345603 | ENA
Project description:The effects of chronic cadmium exposure on gene expression in MCF7 breast cancer cells
| PRJNA227800 | ENA
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.
| S-EPMC2531291 | biostudies-literature
Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.
2019-07-31 | GSE114179 | GEO
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
2011-02-19 | GSE20463 | GEO