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DNA methylation data from normal and chronic periodontitis patients from site OCI and USC

ABSTRACT: DNA methylation data from normal and chronic periodontitis patients from site OCI and USC

PROVIDER: PRJNA257184 | ENA |

REPOSITORIES: ENA

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DNA methylation data from 11 normal and 9 chronic periodontitis patients from site USC

Project description:Microarray methylation (Infinium®HumanMethylation 450 BeadChip from Illumina) was performed on 11 normal and 9 chronic periodontitis (CP) patients

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DNA methylation data from 11 normal and 9 chronic periodontitis patients from site USC

Project description:Microarray methylation (Infinium®HumanMethylation 450 BeadChip from Illumina) was performed on 11 normal and 9 chronic periodontitis (CP) patients Bisulphite converted DNA from the 20 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip

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DNA methylation data from normal and chronic periodontitis patients from site OCI and USC

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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DNA methylation data from normal and chronic periodontitis patients from site OCI and USC

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Transcriptome analysis of chronic periodontitis patients' gingival tissue

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Project description:We report the application of high throughput Illumina sequencing for profiling of small RNAs in saliva of patients who were diagnosed with chronic periodontitis as compared to healthy controls. To date, there is no published literature on salivary microRNA profiling done using the high throughput next-generation sequencing analysis in patients diagnosed with chronic periodontitis. Also, this is the first study of its kind done in an Indian population. The objectives of the study were to profile microRNAs expressed in saliva of patients diagnosed with chronic periodontitis, to identify differentially expressed microRNAs between chronic periodontitis and healthy patients and to identify putative salivary microRNAs which can serve as biomarkers for periodontal disease.

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Proteomic Analysis of Whole Saliva in Chronic Periodontitis

Project description:Periodontitis is a chronic inflammatory disease resulting from a dysbiosis of the dental biofilm and a dysregulated host response in susceptible individuals. It is characterized by periodontal attachment destruction, bone resorption, and eventual tooth loss. Salivary biomarkers have been sought to predict and prevent periodontitis. This comparative study analyzed the salivary proteome of 30 individuals with chronic periodontitis (CP) and 10 with periodontal health (PH), and correlated specific proteins with clinical parameters of disease by using mass spectrometry. Stimulated whole saliva was obtained and pooled for 5 healthy controls and 15 CP patients, precipitated with TCA, digested enzymatically with trypsin and analyzed by an LTQ Orbitrap Velos equipped with a nanoelectrospray ion source. A wide range of salivary proteins of various functions was significantly reduced in CP individuals, whereas salivary acidic proline-rich phosphoprotein, submaxillary gland androgen-regulated protein, histatin, fatty acid-binding protein, thioredoxin, and cystatin were predominant in diseased patients and correlated significantly with signs of periodontal attachment loss and inflammation. Specific salivary proteins were associated with PH and CP. These differences in salivary proteome profiles may contribute to the identification of disease indicators or signatures and the improvement of periodontal diagnosis.

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