Gallus gallus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA258415 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Analysis of genes expressed differentially along the AP and ML axis of chick optic tectum
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Project description:Subtractive cloning of differentially expressed genes in chick heart/hemangioblast precursor cells
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Project description:Newly-hatched domestic chick serves as an important model for studies of neural and behavioral plasticity, particularly with respect to learning and memory such as filial imprinting. Imprinting is assumed to be a unique case of recognition learning with some characteristic features, such as sensitive period and irreversibility. However, the molecules involved in the memory process are yet to be fully identified. To address this issue, we attempted to identify the genes differentially expressed at an earlier phase of filial imprinting than described in our previous report (Brain Res. Bull.76, 275-281 (2008)). One-day-old chicks were trained for imprinting for 1 h and whole brains were collected and used for cDNA microarray analysis and quantitative RT-PCR. We identified 18 genes upregulated accompanying filial imprinting. These results suggested that the increase of these 18 genes associated with filial imprinting might play an important role in the acquisition of memory in the filial imprinting.
2012-08-20 | GSE31055 | GEO
Project description:This analysis aims at finding the microRNAs present in three chick embryo undifferentiated tissues (pools of tissues): Undetermined Presomitic Mesoderm (PSM-U), Determined Presomitic Mesoderm (PSM-D) and Distal limb bud (Limb).
2021-10-25 | E-MTAB-8176 | biostudies-arrayexpress
Project description:Transcriptomic analysis of midbrain and individual hindbrain rhombomeres in the chick embryo
| PRJNA209844 | ENA
Project description:Identification of differentially expressed genes in early inner ear development
| PRJNA117713 | ENA
Project description:Purpose:We have used RNA-seq to examine of differentially expressed miRNAs in chicken leg muscle of three different development stages (11 embryo ages, 16 embryo ages, and 1 day old post hatch chick).The aims of this study are characterization of miRNAs differentially expressed in different developmental stage of chicken embryo, using RNA sequence sample. Methods: On this study we used two embryonic stage and one post hatch chick leg muscle of Xinghua chicken breed. Total RNA from E11 day embryo, E16 day embryo and 1 day post hatch chick was isolated by TRIzol following the manufacturer’s protocol (Invitrogen, CA, USA). Each stages were designed two samples, and the total samples were six (three group × two sample/group) and RNA samples of six individuals were pooled with equal amounts, and then were subjected to Illumina deep sequencing. Results: After eliminating adaptor and low-quality reads, a total of 5,302,700, 6,556,747, 5,359,793, 4,213,112, 7,112,885 and 7,469,939 clean reads were obtained in group E11 (E11.1-E11.2), group E16 (E16.1-E16.2) and group P1 (P1.1–P1.2) libraries, respectively. The clean reads were aligned to the chicken genome databases, miRBase, Rfam, RepBase and mRNA. Conclusions:To assess miRNA expression during chicken embryo skeletal muscle development, we sequenced and analyzed leg muscle at 11 day embryo age, 16 day embryo age, and 1 days post hatch.
2017-06-22 | GSE100321 | GEO
Project description:Newly-hatched domestic chick serves as an important model for studies of neural and behavioral plasticity, particularly with respect to learning and memory such as filial imprinting. Imprinting is assumed to be a unique case of recognition learning with some characteristic features, such as sensitive period and irreversibility. However, the molecules involved in the memory process are yet to be fully identified. To address this issue, we attempted to identify the genes differentially expressed at an earlier phase of filial imprinting than described in our previous report (Brain Res. Bull.76, 275-281 (2008)). One-day-old chicks were trained for imprinting for 1 h and whole brains were collected and used for cDNA microarray analysis and quantitative RT-PCR. We identified 18 genes upregulated accompanying filial imprinting. These results suggested that the increase of these 18 genes associated with filial imprinting might play an important role in the acquisition of memory in the filial imprinting. Total RNA was extracted from whole brains of trained chicks (n=16) and control dark-reared chicks (n=16). Using these total RNAs, we performed RT-PCR to distinguish male chicks from females. Then total RNAs were separated and mixed in four groups (1, male trained (n=8); 2, female trained (n=8); 3, male dark-reared (n=8); and 4, female dark-reared chicks (n=8)), and we performed cDNA microarray expression analysis to identify the upregulated genes following imprinting (1 versus 3 and 2 versus 4).
2012-08-20 | E-GEOD-31055 | biostudies-arrayexpress
Project description:Transcriptome sequencing of thalidomide treated limb bud of chick embryo
| PRJNA318534 | ENA
Project description:In this study, deep sequencing of endometrial RNA samples during early pregnancy were performed using RNA sequencing (RNA-Seq). The changes of genes expressed among three stages (day 12, 18 and 25) of pregnancy were investigated by the multiple comparisons. Analysis of reads, between 1557 and 8951 differentially expressed genes (DEGs) were revealed among different periods of implantation. Expression of selected genes was validated by the use of quantitative real-time RT-PCR. Bioinformatics analysis of differentially expressed genes in the endometrium revealed a number of biological processes and pathways potentially involved in embryo implantation in the pig, most noticeably cell proliferation, regulation of immune response, interaction of cytokine-cytokine receptor, and cell adhesion. This revealed specific gene expression patterns reflecting the different functions of the endometrium on three stages (maternal recognition, conceptus attachment and embryo implantation). This study identified comprehensive transcriptomic profile in the porcine endometrium, it could be a foundation for targeted studies of genes and pathways potentially involved in abnormal endometrial receptivity and embryo loss in early pregnancy.
2015-10-03 | GSE73695 | GEO