Project description:We profiled global gene expression for two separate lines of mouse embryonic fibroblasts and find that deletion of PKM2 and expression of PKM1 does not alter global gene expression profiles. We used microarrays to futher characterize the effects of PKM1 expression compared to PKM2 expression on global gene expression in mouse embryonic fibroblasts. Primary mouse embryonic fibroblast cells were used for RNA extraction and hybridization on Affymetrix microarrays. Intensity files were RMA normalized using Affymetrix expression console.

2015-05-01 | E-GEOD-60499 | biostudies-arrayexpress

Mus musculus

Project description:Expression Data from PtenF341V and Null Mouse Embryonic Fibroblasts

| PRJNA270711 | ENA

Expression data from PKM1 or PKM2 expressing mouse embryonic fibroblasts.

2015-05-01 | GSE60499 | GEO

Mus musculus

Project description:Expression data from human HMGN4- and HMGN2-expressing mouse embryionic fibroblasts

| PRJNA325639 | ENA

Mus musculus

Project description:Expression profiling data of Mta1 from E9.0 mouse embryonic fibroblasts

| PRJNA134737 | ENA

Mus musculus

Project description:Expression data from mouse embryonic fibroblasts and pluripotent stem cells

| PRJNA196638 | ENA

Expression profiling of E14 cells overexpressing Hk2/Pkm2

Project description:Purpose: In this study, we compared transcriptome profiling (RNA-seq) between normal mouse embryonic stem cell (E14) and Hexokinase2 (Hk2)/ Pyruvate Kinase M2 (Pkm2) overexpressed E14 cell. Result: Using an optimized data analysis workflow, we mapped over 4 billion sequence reads per sample to the mouse genome (build mm9) and identified 28698 transcripts in 5 samples. Conclusion: Our study represents the first detailed analysis of Hk2/ Pkm2 overexpressed E14 cell transcriptomes, generated by RNA-seq technology We compared transcriptome profiling (RNA-Seq) between normal mouse embryonic stem cell (E14) and E14 cells over-expressing Hexokinase2 (Hk2)/Pyruvate Kinase M2 (Pkm2)

2016-07-03 | E-GEOD-65246 | biostudies-arrayexpress

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