Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

An alternative pluripotent state confers interspecies chimaeric competency [ChIP-seq]

ABSTRACT: An alternative pluripotent state confers interspecies chimaeric competency [ChIP-seq]

OTHER RELATED OMICS DATASETS IN: GSE60605

PROVIDER: PRJNA258642 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1748450.fastq.gz	Fastqsanger.gz
	SRR1748451.fastq.gz	Fastqsanger.gz
	SRR1748442.fastq.gz	Fastqsanger.gz
	SRR1748452.fastq.gz	Fastqsanger.gz
	SRR1748443.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

An alternative pluripotent state confers interspecies chimaeric competency

Project description:An alternative pluripotent state confers interspecies chimaeric competency

| PRJNA258640 | ENA

An alternative pluripotent state confers interspecies chimaeric competency [RNA-Seq]

Project description:An alternative pluripotent state confers interspecies chimaeric competency [RNA-Seq]

| PRJNA258641 | ENA

Mus musculus

Project description:An alternative pluripotent state confers interspecies chimaeric competency [Microarray]

| PRJNA258464 | ENA

Mus musculus

Project description:An alternative pluripotent state confers interspecies chimaeric competency [WGBS]

| PRJNA268217 | ENA

An alternative pluripotent state confers interspecies chimaeric competency.

Project description:Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture parameters, a stem-cell type with unique spatial characteristics and distinct molecular and functional features, designated as region-selective pluripotent stem cells (rsPSCs), can be efficiently obtained from mouse embryos and primate pluripotent stem cells, including humans. The ease of culturing and editing the genome of human rsPSCs offers advantages for regenerative medicine applications. The unique ability of human rsPSCs to generate post-implantation interspecies chimaeric embryos may facilitate our understanding of early human development and evolution.

| S-EPMC5278765 | biostudies-literature

An alternative pluripotent state confers interspecies chimaeric competency [Microarray]

Project description:Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as intrinsic state-epiblast stem cells (IS-EPIs), could be efficiently derived from different stages of the early embryo. IS-EPIs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed Microarray analysis and compared global gene expression pattern among amplified RNA samples from ESCs, EpiSCS, IS-EPIs as well as in vivo isolated Epiblasts. Examination of global gene expression profiles multiple pluripotent stem cell types

2015-05-06 | E-GEOD-60523 | biostudies-arrayexpress

An alternative pluripotent state confers interspecies chimaeric competency [WGBS]

Project description:In this study, we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed whole-genome bisulfite sequencing (WGBS) experiments to compare the DNA methylation landscapes of conventional EpiSCs and rsEpiSCs. Compare the DNA methylation profiles in 2 pluripotent stem cell types (LP-EpiSCs and conventional EpiSCs) in mouse. Two replicates are examined for each cell type.

2015-05-06 | E-GEOD-63568 | biostudies-arrayexpress

An alternative pluripotent state confers interspecies chimaeric competency

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-05-06 | GSE60605 | GEO

An alternative pluripotent state confers interspecies chimaeric competency [RNA-Seq]

Project description:Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed RNA-sequencing experiments and examined the global gene expression profiles of EpiSCs, rsEpiSCs, in vivo isolated four regions of E6.5 mouse epiblasts: AP (anterior-proximal), AD (anterior-distal), PP (posterior-proximal) and PD (posterior-distal), human H1 ESCs, H1 rsESCs, H9 ESCs, H9 rsESCs, rhesus monkey ORMES23 rsESCs, and chimpanzee rsiPSCs. Examination of global gene expression profiles in 2 pluripotent stem cell types across multiple species.

2015-05-06 | E-GEOD-60603 | biostudies-arrayexpress

An alternative pluripotent state confers interspecies chimaeric competency [ChIP-seq]

Project description:Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed ChIP-seqencing experiments using antibodies against H3K4me3 and H3K27me3, comparing conventional EpiSCs and rsEpiSCs, as well as comparing conventional human H1 ESCs and human H1 ESCs cultured in mouse rsEpiSCs based culture conditions (H1 rsESCs). Examination of 2 different histone modifications (H3K4me3, H3K27me3) in 2 pluripotent stem cell types in mouse and human.

2015-05-06 | E-GEOD-60604 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data