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Campylobacterales

ABSTRACT: Genome sequencing of Helicobacter and Campylobacter species isolated from rodents

PROVIDER: PRJNA261711 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
		Other
	SRR7638880_1.fastq.gz	Fastqsanger.gz
	SRR7638880_2.fastq.gz	Fastqsanger.gz
	SRR7638890_1.fastq.gz	Fastqsanger.gz
	SRR7638890_2.fastq.gz	Fastqsanger.gz

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Dichotomous Regulation of Group 3 Innate Lymphoid Cells by Non-gastric Helicobacter Species from an Immunocompromised Mouse

Project description:Intestinal innate lymphoid cells (ILCs) contribute to the protective immunity and homeostasis of the gut, and the microbiota are critically involved in shaping ILC function. However, the role of the gut microbiota in regulating ILC development and maintenance still remains elusive. Here, we identified opposing effects on ILCs by two Helicobacter species, Helicobacter apodemus and Helicobacter typhlonius, isolated from immunocompromised mice. We demonstrated that the introduction of both Helicobacter species activated ILCs and induced gut inflammation; however, these Helicobacter species negatively regulated RORγt+ Group 3 ILCs (ILC3s), especially T-bet+ ILC3s, and diminished their proliferative capacity. Thus, these findings underscore a previously unknown dichotomous regulation of ILC3s by Helicobacter species, and may serve as a model for further investigations to elucidate the host-microbe interactions that critically sustain the maintenance of intestinal ILC3s.

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Project description:Novel Campylobacter and Helicobacter species isolated from feces of Nile rat (Arvicanthis niloticus)

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Project description:Isolation, Characterization and Antifungal Susceptibility of Cryptococcus species Isolated from rodents

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Nickel Binding- and Histidine-Rich Proteins of Helicobacter species

Project description:The files in this archive examine six species within the genus Helicobacter to assay their histidine-rich proteins. They correspond to the manuscript "Expansion of nickel binding- and histidine-rich proteins during gastric adaptation of Helicobacter species" by Frederic Fischer, Egor Vorontsov, Evelyne Turlin, Christian Malosse, Camille Garcia, David L. Tabb, Julia Chamot-Rooke, Riccardo Percudani, Daniel Vinella and Hilde De Reuse. The sequence databases for the Helicobacter species are based on reference or complete UniProt proteomes in FASTA format, supplemented with reannotations of Hpn and Hpn2 protein products. The full list of RAW files, including their attribution to individual species and the type of experiment they represent, can be found in RAWs-Readme. We have included QuaMeter IDFree metrics to include basic statistics such as length of LC gradient, the number of MS/MS scans, etc.

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Differential RNA-sequencing of Campylobacter jejuni fur perR mutant

Project description:We report the use of differential RNA-sequencing for the determination of the primary transcriptome of the fur perR mutant of Campylobacter jejuni NCTC 11168. This allows for the genome-wide determination of transcription start sites. Campylobacter jejuni NCTC 11168 fur perR mutant was grown to late log phase, RNA was purified and used for differential RNA-sequencing by 454 sequencing with barcoded libraries, and used for determination of genome-wide transcription start sites

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