Project description:Difference in RNA expression levels between Acinetobacter baumannii cells expressing high and low levels of cyclic AMP Total RNA obtained from Acineotbacter baumannii bacterial cells in Log phase gown in MH broth culture, isolated RNA in triplicate from three expreiment. cpdA::Tn mutant and 17978hm strain compared. Assessing increased levels of cAMP within the cell

2015-06-21 | E-GEOD-64935 | biostudies-arrayexpress

Expression Data from Acinetobacter baumannii strain ATCC 17978 and isogenic lpsB mutant cells

Project description:lpsB encodes a glycosyltransferase involved in lipopolysaccharide (LPS) synthesis. LPS is a major component of the Gram-negative bacterial outer membranes. We used custom-made Affymetrix A. baumannii strain ATCC 17978 derived GeneChips to compare the gene expression properties of wild type and isogenic lpsB mutant cells. Two mutants were evaluated; A. baumannii strain 5A7 is a ATCC 17978 derivative harboring a transposon (Tn5) within lpsB (A1S_0430 locus); A. baumannii strain containing a deletion of lpsB (A1S_0430). A. baumannii strain ATCC 17978, 5A7 (lpsB:Tn5) or IH1∆lpsB (∆lpsB) were grown to mid-exponential phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the transcription profile of lpsB mutated cells.

2012-12-01 | E-GEOD-40771 | biostudies-arrayexpress

RNA sequencing of ATCC 17978hm Acineotbacter baumannii strain and CpdA mutant

Project description:Difference in RNA expression levels between Acinetobacter baumannii cells expressing high and low levels of cyclic AMP

2015-06-21 | GSE64935 | GEO