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A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients

ABSTRACT: A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients

PROVIDER: PRJNA262944 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1596230.fastq.gz	Fastqsanger.gz
	SRR1596231.fastq.gz	Fastqsanger.gz
	SRR1596226.fastq.gz	Fastqsanger.gz
	SRR1596227.fastq.gz	Fastqsanger.gz
	SRR1596228.fastq.gz	Fastqsanger.gz

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A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients [NanoString]

Project description:This work highlights similarities and differences between three platforms (next-generation sequencing, microarray and NanoString) for detecting miRNAs and compares their strengths and weaknesses. miRNA expression profiles were determined in Hepatoblastoma FFPE samples using a NanoString platform.

2015-05-03 | E-GEOD-62017 | biostudies-arrayexpress

A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients [microarray]

Project description:This work highlights similarities and differences between three platforms (next-generation sequencing, microarray and NanoString) for detecting miRNAs and compares their strengths and weaknesses. miRNA expression profiles were determined in 6 Hepatoblastoma FFPE samples using a microarray platform.

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A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients [sequencing]

Project description:This work highlights similarities and differences between three platforms (next-generation sequencing, microarray and NanoString) for detecting miRNAs and compares their strengths and weaknesses. miRNA expression profiles were determined in 6 Hepatoblastoma FFPE samples using a next-generation sequencing platform.

2015-05-03 | E-GEOD-62010 | biostudies-arrayexpress

A cross comparison of technologies for the detection of microRNAs in clinical FFPE samples of hepatoblastoma patients.

Project description:Although formalin fixed paraffin embedded (FFPE) tissue is a major biological source in cancer research, it is challenging to work with due to macromolecular fragmentation and nucleic acid crosslinking. Therefore, it is important to characterise the quality of data that can be obtained from FFPE samples. We have compared three independent platforms (next generation sequencing, microarray and NanoString) for profiling microRNAs (miRNAs) using clinical FFPE samples from hepatoblastoma (HB) patients. The number of detected miRNAs ranged from 228 to 345 (median = 294) using the next generation sequencing platform, whereas 79 to 125 (median = 112) miRNAs were identified using microarrays in three HB samples, including technical replicates. NanoString identified 299 to 372 miRNAs in two samples. Between the platforms, we observed high reproducibility and significant levels of shared detection. However, for commonly detected miRNAs, a strong correlation between platforms was not observed. Analysis of 10 additional HB samples with NanoString identified significantly overlapping miRNA expression profiles, and an alternative pattern was identified in a poorly differentiated HB with an aggressive phenotype. This investigation serves as a roadmap for future studies investigating miRNA expression in clinical FFPE samples, and as a guideline for the selection of an appropriate platform.

| S-EPMC4453922 | biostudies-literature

Erratum: A cross comparison of technologies for the detection of microRNAs in clinical FFPE samples of hepatoblastoma patients.

Project description: Not available

| S-EPMC4557124 | biostudies-literature

A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-05-03 | GSE62018 | GEO

A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients [NanoString]

2015-05-03 | GSE62017 | GEO

A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients [microarray]

2015-05-03 | GSE62011 | GEO

A cross-comparison of technologies for the detection of microRNAs from FFPE samples of hepatoblastoma patients [sequencing]