Project description:To evaluate the prognostic relevance of molecular subtypes and key transcription factors in pancreatic ductal adenocarcinoma (PDAC), we performed gene expression analysis of whole-tumor tissue obtained from 118 surgically resected PDAC and 13 control samples.
Project description:We performed RNA-seq analysis on Grem1 wild-type and Grem1-deleted pancreatic tumour tissues from Pdx1-Flp;KrasFSF-G12D;Trp53frt/frt (KPF) mice. We compared the transcriptome between these two groups of tumours with the aim to understand their molecular signatures and the relevance to human pancreatic ductal adenocarcinoma subtypes.
Project description:We performed RNA-seq analysis on Cd61 wild-type and CRISPR/Cas9-mediated Cd61 knock out pancreatic tumour organiod from Pdx1-Flp;KrasFSF-G12D;Trp53frt/frt; Vim-EGFP (KPFV) mice. We compared the transcriptome between these two groups of them with the aim to understand their molecular signatures and the relevance to human pancreatic ductal adenocarcinoma subtypes.
Project description:We performed RNA-seq analysis on Spp1 wild-type and CRISPR/Cas9-mediated Spp1 knock out pancreatic tumour organiod from Pdx1-Flp;KrasFSF-G12D;Trp53frt/frt; Vim-EGFP (KPFV) mice. We compared the transcriptome between these two groups of them with the aim to understand their molecular signatures and the relevance to human pancreatic ductal adenocarcinoma subtypes.
Project description:We generated novel patient derived xenograft (PDX) and cell line -derived xenograft models for pancreatic ductal adenocarcinoma (PDAC) which reflect different molecular subtypes. Pancreatic ductal adenocarcinoma is currently the tumor with the fourth highest mortality rate. Recently, subtypes of PDAC have been reported by Collisson et al (Nat. Med. 17(4) 2011. DOI: 10.1038/nm.2344). However current fetal calf serum (FCS) cultured cell lines do not accurately model these subtypes. We thus generated novel serum-free cell lines derived from primary patient xenografts. We here analyse the gene-expression profiles of the xenografts and the derived cell lines. We show that indeed three different subtypes can be separated in our models based on gene-expression data. Further, we identify upregulation of a drug-detoxification pathway specifically in xenografts and cell lines of one of the subtypes. These models and data will help to better understand inter-patient heterogeneity in PDAC and identify novel drug targets and diagnostic markers.
