Streptococcus mutans UA159
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA266999 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparative proteomic analysis of the biofilm proteome of the wild-type strain, mutant strain, and compound treated strain.
2021-02-26 | PXD021641 | Pride
Project description:The biofilm-forming Streptococcus mutans is a gram-positive bacterium that resides in the human oral cavity and is considered to be the primary etiological agent in the formation of dental caries. The global response regulator CovR, which lacks a cognate sensor kinase, is essential for the pathogenesis and biofilm formation of this bacterium, but it is not clear how covR expression is regulated in S. mutans. In this communication, we present the results of our studies examining various factors that regulate the expression of covR in S. mutans UA159. The results of Southern hybridization and PCR analysis indicated that CovR is an orphan response regulator in various isolates of S. mutans. The transcriptional start site for covR was found to be 221 base pairs upstream of the ATG start codon, and site-directed mutagenesis of the upstream TATAAT box confirmed our findings. The expression of covR is growth phase dependent, with maximal expression observed during exponential-growth phase. While changes to the growth temperature did not significantly affect the expression of covR, increasing the pH or the concentration of Mg(2+) in the growth medium leads to an increase in covR expression. The results of semiquantitative reverse transcriptase PCR analysis and in vivo transcriptional-fusion reporter assays indicated that CovR autoregulates its own expression; this was verified by the results of electrophoretic mobility shift assays and DNase I protection assays, which demonstrated direct binding of CovR to the promoter region. Apparently, regulation by Mg(2+) and the autoregulation of covR are not linked. A detailed analysis of the regulation of CovR may lead to a better understanding of the pathogenesis of S. mutans, as well as providing further insight into the prevention of dental caries.
| S-EPMC2446802 | biostudies-literature
Project description:Global transcriptional analysis of S. mutans metalloregulation
| PRJNA108337 | ENA
Project description:Transcriptional profiling to investigate the roles of ClpP and ClpX of S. mutans
2011-06-10 | GSE29871 | GEO
Project description:Transcriptional profiling to investigate the regulatory roles of SpxA and SpxB of S. mutans.
2009-12-25 | GSE19656 | GEO
Project description:Global transcriptional analysis of S. mutans sugar transporters
| PRJNA99301 | ENA
Project description:Dual regulation of nitrogen metabolism by CodY and GlnR in Streptococcus mutans
| PRJNA110719 | ENA
Project description:Global transcriptional analysis of S. mutans sugar transporters in biofilm
| PRJNA152531 | ENA
Project description:Streptococcus mutans is the leading cause of dental caries (tooth decay) worldwide and is considered to be the most cariogenic of all of the oral streptococci. The genome of S. mutans UA159, a serotype c strain, has been completely sequenced and is composed of 2,030,936 base pairs. It contains 1,963 ORFs, 63% of which have been assigned putative functions. The genome analysis provides further insight into how S. mutans has adapted to surviving the oral environment through resource acquisition, defense against host factors, and use of gene products that maintain its niche against microbial competitors. S. mutans metabolizes a wide variety of carbohydrates via nonoxidative pathways, and all of these pathways have been identified, along with the associated transport systems whose genes account for almost 15% of the genome. Virulence genes associated with extracellular adherent glucan production, adhesins, acid tolerance, proteases, and putative hemolysins have been identified. Strain UA159 is naturally competent and contains all of the genes essential for competence and quorum sensing. Mobile genetic elements in the form of IS elements and transposons are prominent in the genome and include a previously uncharacterized conjugative transposon and a composite transposon containing genes for the synthesis of antibiotics of the gramicidin/bacitracin family; however, no bacteriophage genomes are present.
| S-EPMC137901 | biostudies-literature
Project description:Transcriptional response of Streptococcus mutans in chemically defined medium (CDM) towards the autoinducers CSP and XIP
| PRJNA275633 | ENA