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Streptococcus mutans UA159

ABSTRACT: Transcriptional response of Streptococcus mutans in chemically defined medium (CDM) towards the autoinducers CSP and XIP

PROVIDER: PRJNA275633 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1805910.fastq.gz	Fastqsanger.gz
	SRR1805920.fastq.gz	Fastqsanger.gz
	SRR1805911.fastq.gz	Fastqsanger.gz
	SRR1805921.fastq.gz	Fastqsanger.gz
	SRR1805912.fastq.gz	Fastqsanger.gz

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Transcriptional response of Streptococcus mutans in chemically defined medium (CDM) towards the autoinducers CSP and XIP

Project description:S. mutans UA159 was cultivated in chemically defined medium. Changes in the transcriptome in response to the autoinducing peptides CSP and XIP were monitored for 30 minutes.

2015-06-01 | GSE65982 | GEO

Comprehensive transcriptome profiles distinguish the CSP and XIP signaling pathways in Streptococcus mutans UA159

Project description:In Streptococcus mutans, an oral colonizer associated with dental caries, competence for natural transformation can be triggered by both CSP and XIP pheromones. Competence induced by CSP is a late response that requires induction of the XIP encoding gene comS, but the mechanism(s) linking the two systems remains unknown. To learn how the CSP and XIP pheromone regulatory pathways are temporally linked, we mapped the global changes in gene expression at early and late phases of the CSP response and investigated the effect of deletion of comS on the S. mutans transcriptional profile. The early phase of the CSP response was characterized by an increase in gene expression at five loci associated with bacteriocin production and immunity. In the late phase, the up-regulated regions expanded to include a total of 27 loci, including comS and genes required for DNA uptake and recombination. In the absence of comS, no increase in expression of the genes up-regulated as a late response was observed in response to CSP, whereas expression of those regulated as an early response was maintained. These results indicate that the entire late response to CSP depends on the expression of comS and that the immediate transcriptional response to CSP, mediated by ComE, is restricted to just five bacteriocin-related loci

2015-09-01 | GSE70067 | GEO

Comprehensive transcriptome profiles distinguish the CSP and XIP signaling pathways in Streptococcus mutans UA159

2015-09-01 | E-GEOD-70067 | biostudies-arrayexpress

Streptococcus mutans UA159 control vs. Streptococcus mutans UA159 supplemented with 20 uM 4,5-dihydroxy-2,3-pentadione (DPD) at 3 different pH values

Project description:Transcriptional profiling of early logarithmic phase culture (O.D=0.2-0.3) of Streptococcus mutans UA159 comparing control of untreated Streptococcus mutans UA159 bacteria with Streptococcus mutans UA159 bacteria spplemented with 20µM synthetic DPD (pre-AI-2) which regulates gene expression via AI-2 quorum sensing system.Three compairisons were performed at pHs of 7,6 and 5.

2010-07-29 | GSE23159 | GEO

Transcriptome analysis of Streptococcus mutans quorum sensing-induced cells

Project description:In the human pathogen Streptococcus mutans, the canonical peptide-based quorum sensing system is an inducible DNA repair system that is pivotal for bacterial survival. Previous work showed that the CSP signaling peptide is a stress-signaling alarmone that controls different stress-induced phenotypes. In this study, we exposed S. mutans to the CSP pheromone to mimic DNA damage conditions. Transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary phase cells and the CSP-induced stationary phase cells. The data obtained contribute to the understanding of the CSP-induced phenotypes in S. mutans.

2024-04-28 | GSE264670 | GEO

Transcriptomic comparison of D39 WT and SPD_0588 deletion mutant in Streptococcus pneumoniae D39 with 1 µg/ml methionine

Project description:metR (SPD_0588) mutant (TH9197) cannot grow in chemically defined medium (CDM) without methionine and had significant growth defect in CDM with 1 µg/ml methionine. With the increasing concentration of mehtionine, the growth of TH9197 was restored and the growth of metR mutant is as same as D39 WT (TH4306) when the concentration of methionine reaches 50 µg/ml. SPD_0588 is a putative transcriptional regulator and is homologous to MetR in Streptococcus mutans (78.9 % amino acid identity), which regulates both methionine synthesis and transport. We compared the transcriptome of WT and metR mutant in CDM with 1 µg/ml methionine by RNA-seq to see if MetR regulates the metabolism of methionine in Streptococcus pneumoniae D39. We also want to identify the regulon of SPD_0588.

2021-06-01 | GSE142362 | GEO

Dual regulation of nitrogen metabolism by CodY and GlnR in Streptococcus mutans

Project description:CodY and GlnR are two major transcriptional regulators in nitrogen metabolism in Gram-positive bacteria. CodY regulates genes involved in the adaptive response to poor growth conditions, especially to nutrient limitation. GlnR controls nitrogen utilization according to the availability of nitrogen source. In this study we used microarray to investigate the regulatory roles that CodY and GlnR play in nitrogen metabolism in Streptococcus mutans. Streptococcus mutans UA159 wild-type cells, ΔcodY, ΔglnR, and ΔcodYglnR strains were grown in a chemically-defined medium until the mid-log phase. The nitrogen source was 1% tryptone. Twenty millimolar sodium thiosulfate was added to the medium to support cysteine biosynthesis. The transcriptional profile of the whole genome was examined with microarray.

2010-07-01 | E-GEOD-13540 | biostudies-arrayexpress

Identification of the phosphotransacetylase (pta) and acetate kinase (ackA) regulons in Streptococcus mutans UA159

Project description:Transcriptional comparisons between wild-type Streptococcus mutans UA159 and two mutant strains (M-bM-^HM-^FackA and M-bM-^HM-^FackA pta). On growth condition in defined FMC medium, WT vs. either M-bM-^HM-^FackA or M-bM-^HM-^FackA pta mutants. Biological replicates: 10 control, 5 each mutant, independently grown and harvested. One replicate per array.

2013-05-11 | E-GEOD-46835 | biostudies-arrayexpress

Streptococcus mutans UA159

Project description:Comprehensive transcriptome profiles distinguish the CSP and XIP signaling pathways in Streptococcus mutans UA159

| PRJNA287561 | ENA

Arginine-specific effects on the transcriptome of Streptococcus mutans probed by RNA-seq

Project description:Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In this study, we observed supplementation with 1.5% arginine (final concentration) had inhibitory effects on the growth of S. mutans in complex and chemically defined media, particularly when cells were exposed to acid or oxidative stress. Deep-sequencing of RNA (RNA-Seq) comparing the transcriptomes of S. mutans growing in a chemically defined medium with and without 1.5% arginine in neutral and acidic pH conditions and under oxidative stress conditions revealed interesting results. The results provide new insights into the mechanisms of action by which arginine inhibits dental caries through direct adverse effects on multiple virulence-related properties of the most common human dental caries pathogen. The findings significantly enhance our understanding of the genetics and physiology of this cariogenic pathogen.

2017-05-20 | GSE98020 | GEO

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