Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA267660 | ENA |
REPOSITORIES: ENA
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Project description:The long non-coding RNA NUDT6 was found to be deregulated in abdominal aortic aneurysm (AAA) with higher expression in diseased human tissue specimens versus control aortic tissue. Apart from the already well-studied DNA: RNA interaction as a natural antisense transcript to Fibroblast Growth Factor 2 (FGF2), we were interested in identifying protein interaction partners to unravel further involvement in the pathogenesis and progression of abdominal aortic aneurysm. Therefore, we performed a RNA pulldown experiment using biotinylated NUDT6 and control RNA in human aortic smooth muscle cell lysate to identify further interaction partners.
2024-01-26 | PXD033922 | Pride
Project description:Abdominal aortic aneurysm (AAA) is a permanent segmental dilatation of the abdominal aorta, contributing to a high mortality once rupture. We performed RNA-sequencing analysis of abdominal aorta tissues from 14 participants, including seven patients with AAA and seven control individuals.
2023-12-07 | GSE183464 | GEO
Project description:The aim of this study was to assess the relative gene expression in human AAA and AOD. Genome-wide expression analysis of abdominal aortic aneurysm (AAA) and aortic occlusive disease (AOD) specimens obtained from 20 patients with small AAA (mean maximum aortic diameter=54.3±2.3 mm), 29 patients with large AAA (mean maximum aortic diameter=68.4±14.3 mm), and 9 AOD patients (mean maximum aortic diameter=19.6±2.6 mm). Relative aortic gene expression was compared with that of 10 control aortic specimen of organ donors.
2015-06-30 | E-GEOD-57691 | biostudies-arrayexpress
Project description:Background: Abdominal aortic aneurysm (AAA) is a vascular disease with indeterminate prevalence but high mortality rates when complicated with rupture. The pathogenesis of AAA has not been fully elucidated. N6-methyladenosine (m6A) modification is likely important in the development of AAA. In the present study, m6A-MeRIP sequencing and RNA sequencing were performed to identify the m6A sites. Bioinformatics analysis was used to evaluate the m6A patterns of the aorta walls of AngII-induced abdominal aortic aneurysm (AAA) model and normal mice. Results: There were 2039 differentially methylated m6A peaks involving 1865 mRNAs in the AAA group relative to the control, of which 1610 peaks in 1466 mRNAs were hypermethylated and 429 peaks in 410 mRNAs were hypomethylated. The hypermethylated mRNAs in AAA group were mostly enriched in transcription regulation and intercellular signaling, especially the Wnt signaling-associated processes. Hypomethylated m6A sites were mainly enriched in G protein-coupled receptor activity and ion channel activity. Conclusion: Our study suggested an original viewpoint that AAA might mainly be relevant to combined effect of m6A methylation modification in Wnt pathway, G protein-coupled receptor and ion channel- associated genes, which were worthy of further investigation.
2021-04-09 | GSE171371 | GEO
Project description:In this study we used microarrays to examine relative genes expression within the aorta of ApoE-/- infused with angiotensin II in relation to aneurysm formation. Infusion of angiotensin II induces aortic dilatation particularly of the suprarenal aorta in ApoE-/- mice. Based on studies carried out in our and other laboratories the response to angiotensin II is variable, with some mice developing large aneurysms but other animals appearing resistant to aneurysm formation with aortic diameters similar to that of saline controls. We compared RNA expression from whole aortas of 17 week old male ApoE-/- mice exposed to angiotensin II (1.44 µg/kg/min) for 4 weeks where there was clear evidence of aortic aneurysm formation (n=5) with that of mice failing to develop aneurysms (n=7) and those exposed to saline infusion (n=6). AAA was defined as diameter of suprarenal aorta greated than 1.5mm measured on photographs of aortas at necroscopy. Keywords: Disease state analysis 18 samples analysed, AAA (n=5), no AAA (n=7), saline (n=6). AAA - abdominal aortic aneurysm
2010-06-24 | E-GEOD-12591 | biostudies-arrayexpress
Project description:The ApoE -/- mice model of abdominal aortic aneurysm (AAA) involves introducing Angiotensin II subcutaneously to 14 week old male mice for 4 weeks by osmotic pump. A significant number of mice will develop aneurysm-like dilations in the suprarenal section of the abdominal aorta (SRA) that have a number of similarities to the human condition and make this a useful model of AAA. The mouse infrarenal aorta is very resistant to aneurysm formation while in humans AAA predominately occurs in the infrarenal section of the aorta (IRA). There have been a number of theories proposed to explain the site selctivity of aneurysm formation in AAA and this mice model. This study was designed to ascertain differences between SRA and IRA that may explain this site selectivity. Keywords: tissue type comparison
2010-05-12 | GSE7006 | GEO
Project description:Inflammation is still a crucial factor in the development of abdominal aortic aneurysm (AAA). The CD45+ cell population of elastase-induced murine AAA was deconstructed at the single-cell level using the single-cell RNA (scRNA) transcriptomic technique.
2023-07-18 | GSE237067 | GEO
Project description:We report the transcriptomic analysis of RNA-seq of abdominal aortas isolated from ApoE-/- and ApoE-/-Nur77-/- mice mice exposed to Ang II to uncover the mechanisms underlying the undefined role of Nur77 in abdominal aortic aneurysm (AAA)
2021-05-21 | GSE174768 | GEO
Project description:mRNA and Long noncoding RNA profiles in the mouse abdominal aorta with AAA and without AAA.
2017-08-01 | GSE63396 | GEO
Project description:Abdominal aortic aneurysm (AAA) is a lethal disease, occurring mostly in men more than 65 years of age. Until recently, the pathogenesis of AAA remains poorly understood. MicroRNAs (miRNAs) are a novel class of endogenous small noncoding RNAs that play important roles in diverse biological and pathological processes and was more recently investigated in cardiovascular physiology and pathology. In this study, we employed microarray to detect and compare miRNA expressions of AAAs in rats. Four miRNAs were validated using real time RT-PCR. Functional annotations of putative targets of deregulated miRNAs via bioinformatics approaches revealed that predicted targets were highly enriched and involved in several signaling pathways important for AAA formation. Our results indicate that miRNAs are extensively involved in rat AAA formation and provide a global view of AAA miRNA profiles, which is expected to provide new clues to develop targeted therapies against this calamitous disease. Sprague-Dawley rat AAA model was established by calcium chloride and collagenase co-incubation method. After 28 days, three rats with confirmed AAAs and three normal rats form control(sham operation) group were euthanized and the aortas specimens were used for this experiment.
2010-09-18 | E-GEOD-24194 | biostudies-arrayexpress