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ABSTRACT: Genome-wide analysis of wild type and gemin2 mutant plants exposed to cold

OTHER RELATED OMICS DATASETS IN: E-GEOD-63406E-GEOD-63405

PROVIDER: PRJNA267678 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1655100.fastq.gz	Fastqsanger.gz
	SRR1655110_1.fastq.gz	Fastqsanger.gz
	SRR1655110_2.fastq.gz	Fastqsanger.gz
	SRR1655120_1.fastq.gz	Fastqsanger.gz
	SRR1655120_2.fastq.gz	Fastqsanger.gz

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Project description:Cold exposure leads to alteration in the structure of the male sex chromosome of the mutant strain In(1)BM2(reinverted). Genome wide expression profiling was used to identify candidate genes involved in the expression of this phenotype. Adult male flies were exposed to cold shock at 12±1°C for 4 hours and the differentially expressed genes in the strain was compared to similarly exposed wild type Oregon R males. The microarray data was further validated using real time PCR.

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Wheat mutant “RYNO3936” is associated with delayed drought-induced leaf senescence and rapid drought recovery

Project description:Random mutagenesis was applied to produce a new wheat mutant (RYNO3926) with superior characteristics regarding tolerance to water deficit treatment and rapid recovery from water stress conditions. Under water stress conditions mutant plants reached maturity faster and produced more seeds than its wild type wheat progenitor Further, whereas wild-type Tugela DN plants died within 7 days after induction of water stress, mutant plants survived by maintaining a higher relative moisture content (RMC), increased total chlorophyll, a higher photosynthesis rate and stomatal conductance suggesting that the mutant may possess a “stay green”’ phenotype. Analysis of the proteome of mutant plants revealed that they better regulate post-translational modification (SUMOylation) and have increased expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) proteins. Mutant plants also expressed unique proteins associated with dehydration tolerance including abscisic stress-ripening protein, cold induced protein, cold-responsive protein, dehydrin, Group 3 late embryogenesis and also a lipoprotein (LAlv9) belonging to the family of lipocalins. Overall, our results suggest that our new mutant RYNO3936 has a potential for inclusion in future breeding programs to improve drought tolerance under dryland conditions.

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In(1)BM2(reinverted) males: reared at room temperature vs. Cold shock treatment

Project description:Cold exposure leads to alteration in the structure of the male sex chromosome of the mutant strain In(1)BM2(reinverted). Genome wide expression profiling was used to identify candidate genes involved in the expression of this phenotype. Adult male flies were exposed to cold shock at 12Â±1Â°C for 4 hours and the differentially expressed genes in the strain was compared to similarly exposed wild type Oregon R males. The microarray data was further validated using real time PCR. Two-condition experiment, RT vs. CS flies. Biological replicates: 2 control replicates, 2 replicates exposed to cold shock.

2015-11-05 | E-GEOD-74673 | biostudies-arrayexpress

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