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Oryzias melastigma

ABSTRACT: Marine medaka Whole Transcriptome analysis

PROVIDER: PRJNA268138 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1659084_1.fastq.gz	Fastqsanger.gz
	SRR1659084_2.fastq.gz	Fastqsanger.gz
	SRR1658888_1.fastq.gz	Fastqsanger.gz
	SRR1658888_2.fastq.gz	Fastqsanger.gz
	SRR1658869_1.fastq.gz	Fastqsanger.gz

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Project description:Identification and expression profiling of microRNAs in brain, liver and gonads of marine medaka (Oryzias melastigma) by Illumina small RNA Sequencing

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Identification and expression profiling of microRNAs in brain, liver and gonads of marine medaka (Oryzias melastigma) by Illumina small RNA Sequencing

Project description:We performed miRNA transcriptome analysis of mature medaka tissues using high-throughput next generation sequencing technology. The small RNA libraries were contructed from brain, liver and gonads in mature male and female medaka and a total of more than 128 million sequencing reads were generated from the six small RNA libraries. By comparison to known miRNA database, a total of 223 known miRNA types were identified with more than 50% expressed in brain. The expression profiling showed that almost half of the miRNAs are tissue-specific, with only 55 miRNA types from 34 families common to all tissues. A small number of miRNAs are also gender-specific. The experimental validation was performed for five represented miRNA candidates and four of them are confirmed.

2014-07-01 | GSE42696 | GEO