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Oryzias melastigma

ABSTRACT: Hypoxia causes gender-specfic hepatic toxicity in marine medaka

PROVIDER: PRJNA578030 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10353243.fastq.gz	Fastqsanger.gz
	SRR10353244.fastq.gz	Fastqsanger.gz
	SRR10353245.fastq.gz	Fastqsanger.gz
	SRR10353246.fastq.gz	Fastqsanger.gz
	SRR10353247.fastq.gz	Fastqsanger.gz

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Identification and expression profiling of microRNAs in brain, liver and gonads of marine medaka (Oryzias melastigma) by Illumina small RNA Sequencing

Project description:We performed miRNA transcriptome analysis of mature medaka tissues using high-throughput next generation sequencing technology. The small RNA libraries were contructed from brain, liver and gonads in mature male and female medaka and a total of more than 128 million sequencing reads were generated from the six small RNA libraries. By comparison to known miRNA database, a total of 223 known miRNA types were identified with more than 50% expressed in brain. The expression profiling showed that almost half of the miRNAs are tissue-specific, with only 55 miRNA types from 34 families common to all tissues. A small number of miRNAs are also gender-specific. The experimental validation was performed for five represented miRNA candidates and four of them are confirmed.

2014-07-01 | GSE42696 | GEO

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Project description:Identification and expression profiling of microRNAs in brain, liver and gonads of marine medaka (Oryzias melastigma) by Illumina small RNA Sequencing

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MeDIP on the marine medaka sperm (Oryzias melastigma)

Project description:Hypoxia is amongst the most widespread and pressing problems in aquatic environments. For the first time, we demonstrate that fish (Oryzias melastigma) exposed to hypoxia show reproductive impairments in F2 generations despite these progenies (and their germ cells) having never been exposed to hypoxia. We further show that the observed transgenerational reproductive impairments are associated with a differential methylation pattern of specific genes in sperm of both F0 and F2.

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