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Bacillus subtilis subsp. subtilis str. 168

ABSTRACT: Altered germination properties of Bacillus subtilis 168 strains

PROVIDER: PRJNA268543 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR1663457_1.fastq.gz	Fastqsanger.gz
	SRR1663457_2.fastq.gz	Fastqsanger.gz
	SRR1663467_1.fastq.gz	Fastqsanger.gz
	SRR1663467_2.fastq.gz	Fastqsanger.gz

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RNA-seq established gene expression data to determine the effects of various stresses on Bacillus subtilis str.168

Project description:To explore the effects of different stress conditions on Bacillus subtilis str.168, a selection of conditions were applied to the organism and RNA-seq data gathered. A matrix of gene counts was produced as a basis for further analysis into the transcription profiles of Bacillus subtilis str.168.

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Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene

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Project description:Transcriptional response of Bacillus subtilis to moenomycin in wild-type 168. Bacillus subtilis 168, WT (-MOE) vs. WT (+MOE). The experiment was conducted in triplicate using three independent total RNA preparations. Untreated samples were labeled with Alexa Fluor 555 and moenomycin treated samples were labeled with Alexa Fluor 647.

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Project description:Identification of the specific WalR (YycF) binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the WalRK regulon in Bacillus subtilis and its role in cell wall metabolism, as well as implying a role in several other cellular processes. For each sample analyzed in this study three biological replicates were performed. Three different samples were taken from a strain expressing the WalR-SPA protein as well as from wild-type (168) without a tagged WalR. Samples were taken from exponentially growing cells in low phosphate medium (LPDM) as well as from phosphate-limited cells (T2). Each sample compares ChIP DNA vs. Total DNA from the same cells.

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