Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Saccharomyces cerevisiae

ABSTRACT: Genome wide maps of replicative polymerase contributions to the fully replicated yeast genome

PROVIDER: PRJNA271170 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1734970.fastq.gz	Fastqsanger.gz
	SRR1734980.fastq.gz	Fastqsanger.gz
	SRR1734971.fastq.gz	Fastqsanger.gz
	SRR1734981.fastq.gz	Fastqsanger.gz
	SRR1734972.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Genome wide maps of replicative polymerase contributions to the fully replicated yeast genome

Project description:We report the application of high through-put tag sequencing to measure the location and strand of DNA embedded ribonucleotides in the yeast genome. Mutations in the catalytic subunits of the polymerases (pol1-L868M, pol2-M644G and pol3-L612M) lead to the increased incorporation of ribonucleotides during DNA replication, providing an in vivo label with which to track the contribution of each polymerase to the fully replicated genome. Yeast strains used in this study are deleted for rnh201, encoding the catalytic subunit of the RNase H2 gene so that embedded ribonucleotides are not rapidly removed by ribonucleotide excision repair following DNA replication. Analysis of this data demonstrates that polymerase alpha contributes to the fully replicated genome. Sequencing of DNA embedded ribonucleotides in S. cerevisiae strains to map the contribution of replicative polymerases to the fully replicated genome.

2015-01-26 | E-GEOD-64521 | biostudies-arrayexpress

Saccharomyces cerevisiae

Project description:Genome-wide mapping of yeast RNA polymerase II termination

| PRJNA243299 | ENA

Genome-wide maps of chromatin state in saccharomyces cerevisiae

Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in yeast. By obtaining bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of saccharomuces cerevisiae.We find that H3T11 phosphorylationlysine is widely distributed in gene promoter region and chromosome telomere region

2020-03-12 | GSE146821 | GEO

Saccharomyces cerevisiae

Project description:Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase

| PRJNA100605 | ENA

Saccharomyces cerevisiae

Project description:Open chromatin maps of genetically different yeast strains

| PRJNA148785 | ENA

Saccharomyces cerevisiae

Project description:Genome-wide Maps of Alkylation Damage, Repair, and Mutagenesis in Yeast Reveal Mechanisms of Mutational Heterogeneity

| PRJNA383738 | ENA

Saccharomyces cerevisiae

Project description:Genome-wide maps of nucleosome positions in Saccharomyces strains bearing heterologous DNA on yeast artificial chromosomes

| PRJNA169603 | ENA

Pathways change in expression during replicative aging in Saccharomyces cerevisiae

Project description:Yeast replicative aging is a process resembling replicative aging in mammalian cells. During aging, wild type haploid yeast cells enlarge, become sterile, and undergo nucleolar enlargement and fragmentation; we sought gene expression changes during the time of these phenotypic changes. Gene expression studied via microarrays and qPCR has shown reproducible, statistically significant changes in mRNA of genes at 12 and 18-20 generations. Our findings support previously described changes towards aerobic metabolism, decreased ribosome gene expression, and a partial Environmental Stress Response. Our novel findings include a pseudo-stationary phase, down-regulation of methylation-related metabolism, increased Nucleotide Excision Repair related mRNA, and a strong up-regulation of many of the regulatory subunits of protein phosphatase I (Glc7). These findings are correlated with aging changes in higher organisms as well as with the known involvement of protein phosphorylation states during yeast aging. J Gerontol, Jan, 2008, vol 63A, no. 1. Keywords: aging time course

2008-02-01 | GSE10018 | GEO

Aneuploidy shortens replicative lifespan in Saccharomyces cerevisiae

Project description:Aneuploidy and aging are correlated; however, a causal link between these two phenomena has remained elusive. Here we show that yeast disomic for a single native yeast chromosome generally have a decreased replicative lifespan. In addition, the extent of this lifespan deficit correlates with the size of the extra chromosome. We identified a mutation in BUL1 that rescues both the lifespan deficit and a protein trafficking defect in yeast disomic for chromosome 5. Bul1 is an E4 ubiquitin ligase adaptor involved in a protein quality-control pathway that targets membrane proteins for endocytosis and destruction in the lysosomal vacuole thereby maintaining protein homeostasis. Concurrent suppression of the aging and trafficking phenotypes suggests that disrupted membrane protein homeostasis in aneuploid yeast may contribute to their accelerated aging. The data reported here demonstrate that aneuploidy can impair protein homeostasis, shorten lifespan, and may contribute to age-associated phenotypes.

2015-12-04 | GSE75623 | GEO

Saccharomyces cerevisiae

Project description:Nucleosome loss leads to global transcriptional upregulation and genomic instability during yeast replicative aging

| PRJNA203300 | ENA