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Callithrix jacchus

ABSTRACT: Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

PROVIDER: PRJNA272606 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1758440.fastq.gz	Fastqsanger.gz
	SRR1758441.fastq.gz	Fastqsanger.gz
	SRR1758442.fastq.gz	Fastqsanger.gz
	SRR1758443.fastq.gz	Fastqsanger.gz
	SRR1758434.fastq.gz	Fastqsanger.gz

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Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

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Non-viral generation of marmoset monkey iPS cells by a six-factor-in-one-vector approach

Project description:The analysis compares primary fibroblasts initially used for reprogramming, established marmoset ES cells and a marmoset iPS cell line which was generated witha non-viral approach using a six-factor-in-one-vector approach Cells were grown under standard conditions for marmoset pluripotent stem cells and primary fibroblasts without additional treatment. For each cell type at least four independent cell preparations were used.

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Establishing a common marmoset induced pluripotent stem cell line NM-iPS by an all-in-one episomal vector approach

Project description:Epstein-Barr virus (EBV)-based episomal vector system enables persistent transgene expression, which is advantageous for efficient derivation of transgene-free induced pluripotent stem cells (iPSCs) without viral transduction. Here, we report establishment of an iPSC line from somatic fibroblasts of a neonatal common marmoset monkey (marmoset; Callithrix jacchus) using an all-in-one episomal vector that we newly developed. The established iPSC line, named NM-iPS, showed standard characteristics of pluripotency such as pluripotency-related marker expression, three germ layer differentiation, and normal karyotype (2n = 46). The novel iPSC line would be a useful resource for stem cell research using non-human primates.

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Project description:RNA sequencing, expression of the marmoset PGCLCs, iPS cells and PGCLC aggregates were subject to single-cell RNA-seq analyses.

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Project description:mRNA sequence of common marmoset brain

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