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Pseudomonas syringae pv. maculicola strain:CFBP 1657

ABSTRACT: Pseudomonas syringae pv. maculicola strain:CFBP 1657 Genome sequencing and assembly

PROVIDER: PRJNA274689 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	JYHH01.dat.gz	Other
	JYHH01.fasta.gz	Fasta.gz
	JYHH01.master.dat	Other
	JYHH01.dat.gz	Other

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Project description:Phytoalexins are abundant in edible crucifers and have important biological activities, yet no dedicated gene for their biosynthesis is known. Here, we report two new cytochromes P450 from the non-model plant Brassica rapa (Chinese cabbage) that catalyze unprecedented S-heterocyclizations in cyclobrassinin and spirobrassinin biosynthesis. Our results reveal the first genetic and biochemical insights into the biosynthesis of a prominent pair of dietary metabolites, and have important implications for pathway discovery across >20 recently sequenced non-model crucifers. Leaf mRNA profiles of four conditions (Pseudomonas syringae pv. maculicola, flg22, and their respective mock treatments) were tested in triplicate.

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