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Methanosarcina barkeri str. Fusaro

ABSTRACT: Pyr+ Methanosarcina barkeri Fusaro

PROVIDER: PRJNA275150 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2064280.fastq.gz	Fastqsanger.gz
	SRR2064282_1.fastq.gz	Fastqsanger.gz
	SRR2064282_2.fastq.gz	Fastqsanger.gz
	SRR2064286_1.fastq.gz	Fastqsanger.gz
	SRR2064286_2.fastq.gz	Fastqsanger.gz

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Project description:The goal of this work was to elucidate the mechanism by which pyruvate is utilized as a substrate in a mutant strain of Methanosarcina barkeri Fusaro. In this study, using RNAseq we gained insight into how the mutant strain modulate its transcriptional profile in order to use pyruvate as a substrate. In addition, we obtained information on how methanogens respond to pyruvate at the transcriptional level. The mRNA from of Methanosarcina barkeri Fusaro DSMZ804 and Pyr+ strains grown on a variety of substrates (methanol, acetate, methanol-acetate, methanol-pyruvate, methanol-pyruvate-acetate) were harvested sequenced and mapped to M. barkeri genome. Pairwise comparisons between two cell lines of the Pyr+ strain and the DSMZ 804 strain were performed in all substrates tested.

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