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Genomic maps of TUG1 and gene expression profiling in human glioma stem cell

ABSTRACT: Genomic maps of TUG1 and gene expression profiling in human glioma stem cell

PROVIDER: PRJNA275381 | ENA |

REPOSITORIES: ENA

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Genomic maps of TUG1 in human glioma stem cell

Project description:Glioblastomas show heterogeneous histological features. These distinct phenotypic states are thought to be originated by the glioma stem cells (GSCs), which are highly tumorigenic and self-renewing sub-population of tumor cells that have different functional characteristics. We found TUG1, a large non-coding RNA (lncRNA) is highly expressed in GSCs. TUG1 was reported to interact with PRC2 via its exon 2 and represses its target gene expression in trans. In order to identify the genetic loci enriched with TUG1 in GSC, we performed modified RNA pull-down assay coupled with promoter-microarray analysis using BrU-labeled TUG1.

2016-11-23 | GSE65910 | GEO

miRNA expression profiling in human glioma stem cell upon TUG1 inhibition

Project description:Glioblastomas show heterogeneous histological features. These distinct phenotypic states are thought to be associated with the presence of glioma stem cells (GSCs), which are highly tumorigenic and self-renewing sub-population of tumor cells that have different functional characteristics. We found that TUG1, a long non-coding RNA (lncRNA), plays pivotal roles in maintaining the self-renewal properties of GSC. Some lncRNAs are known to act as a miRNA sponge in the cytoplasm, where lncRNAs bind to miRNAs and quench their activity. To investigate miRNA expression profiling in GSC upon TUG1 inhibition, we have performed microarray experiment using SurePrint G3 Human miRNA 8x60K Microarray (G4872A, Agilent Technologies).

2016-11-23 | GSE79896 | GEO

Genomic maps of TUG1 and gene expression profiling in human glioma stem cell

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-11-23 | GSE65911 | GEO

Homo sapiens

Project description:Genomic maps of TUG1 in human glioma stem cell

| PRJNA275382 | ENA

Aging-regulated TUG1 is dispensable for endothelial cell function

Project description:The evolutionary conserved Taurine Upregulated Gene 1 (TUG1) is a ubiquitously expressed gene that is one of the highest expressed genes in human and rodent endothelial cells (ECs). We here show that TUG1 expression decreases significantly in aging mouse carotid artery ECs and human ECs in vitro, indicating a potential role in the aging endothelial vasculature system. We therefore investigated if, and how, TUG1 might function in aging ECs, but despite extensive phenotyping found no alterations in basal EC proliferation, apoptosis, barrier function, migration, mitochondrial function, or monocyte adhesion upon TUG1 silencing in vitro. TUG1 knockdown did slightly and significantly decrease cumulative sprout length upon vascular endothelial growth factor A stimulation in human umbilical vein endothelial cells (HUVECs), though TUG1-silenced HUVECs displayed no transcriptome-wide mRNA expression changes explaining this effect. Further, ectopic expression of the highly conserved and recently discovered 153 amino acid protein translated from certain TUG1 transcript isoforms did not alter angiogenic sprouting in vitro. Our data show that, despite a high expression and strong evolutionary conservation of both the TUG1 locus and the protein sequence it encodes, TUG1 does not seem to play a major role in basic endothelial cell function.

2022-07-20 | GSE200766 | GEO

Genome-wide RNA binding landscapes of PABPC1 and CCT3 in GC-2spd(ts) and verification of their direct interactions with lncRNA Tug1

Project description:Verification of direct interactions between lncRNA Tug1 and RNA-binding proteins PABPC1 and CCT3 and genome-wide RNA binding maps of PABPC1 and CCT3 in GC-2spd(ts)

2026-06-11 | GSE334383 | GEO

The Tug1 locus is required for male fertility and comprised of DNA, RNA and Protein regulatory elements

Project description:We have sequenced mouse embryonic fibroblasts (MEFs) and 6 organs (testes, prostate, liver, heart, spleen, and eye) harvested from adult male wild type and Tug1 knockout (Tug1_tm1.1Vlcg) mice in a C57BL/6J/129S6 (N3-C57BL/6J, no Neo) background. Additional sequencing was done on the testes of a Tug1 rescue mouse, which contains a doxycycline inducible allele for Tug1 (tg(Tug1)) and CAGs-rtTA3 in the Tug1 knockout background (Tug1_tmn1.1Vlcg).

2020-05-27 | GSE124745 | GEO

Genome wide maps of H3K4me3 and H3ac histone modifications in C6 rat glioma cells [Illumina]

Project description:We report maps of H3K4me3 and H3ac - activiting expression histone modifications in C6 rat glioma cells. The data was obtained using whole genome high throughput technology. The sequencing was performed on HiSeq Ilumina platform. Examination of H3K4me3 histone modification and H3ac histone modification in C6 rat glioma cell line

2015-05-07 | E-GEOD-46820 | biostudies-arrayexpress

Gene expression profiling and genome-wide maps in PDGFB-induced glioma

Project description:Gene expression profiling and genome-wide maps in PDGFB-induced glioma

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Cellular heterogeneity and therapeutic response profiling of IDH-wildtype glioma stem cell cultures

Project description:Gliomas are the most common type of primary malignant adult brain tumor. They appear to originate from neuroglial stem or progenitor cells, and are therapeutically challenging due to an invasive growth pattern and the absence of effective therapies. We have analyzed cellular, molecular and proteomic features and defined the therapeutic response profiles of four IDH1-wildtype glioma stem cell (GSC) cultures. All four GSC cultures were established from Grade IV glioblastoma (GBM) surgical resection tissue, can be continuously propagated and are highly enriched for stem/tumor repopulating cells. Integrated genomic and proteomic analysis of all four cultures was performed, together with use of a dual molecular bar-coding strategy to assess GSC population heterogeneity and the response to ionizing radiation. These well-characterized, bar-coded GSC cultures provide an experimentally tractable resource for investigating glioma biology, and to use to identify new and potentially more effective GBM therapies and treatment regimens.

2026-02-02 | PXD035886 | Pride

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