Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiling and genome-wide maps in PDGFB-induced glioma

ABSTRACT: Gene expression profiling and genome-wide maps in PDGFB-induced glioma

PROVIDER: PRJNA317782 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR2133140.fastq.gz	Fastqsanger.gz
	SRR2133141.fastq.gz	Fastqsanger.gz
	SRR2133142.fastq.gz	Fastqsanger.gz
	SRR2133143.fastq.gz	Fastqsanger.gz
	SRR2133134.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Genome-wide maps in PDGFB-induced glioma

Project description:We performed genome-wide profiling of oligodendrocyte lineage transcription factor 2 (Olig2) and other histone markers in platelet-derived growth factor subunit B (PDGFB)-induced glioma and genome-occupancy analyses coupled with transcriptome profiling to reveal gene regulatory network. Examination of Olig2, H327Ac, and H3K4me3 genome-wide occupancy in PDGFB-induced Ctrl-T and Olig2cKO brain tumors (gliomas).

2016-05-17 | E-GEOD-80088 | biostudies-arrayexpress

Genome-wide maps in PDGFB-induced glioma

2016-05-17 | GSE80088 | GEO

Gene expression profiling of retrovirus PDGFB-induced glioma

2016-05-17 | GSE80087 | GEO

Gene expression profiling of retrovirus PDGFB-induced glioma

Project description:We performed gene expression profilings of platelet-derived growth factor subunit B (PDGFB)-induced mouse glioma to compare the differential transcriptome profiles between Ctrl-T tumor cells and Olig2cKO tumor cells. Expression profiling of Ctrl-T and Olig2cKO brain tumor (glioma) cells, normal oligodendrocyte progenitor cells (OPCs), normal astrocytes, and normal brain cortex by high-throughput sequencing.

2016-05-17 | E-GEOD-80087 | biostudies-arrayexpress

Mutant IDH impairs chromatin binding by PDGFB to promote chromosome instability

Project description:Non-canonical roles for growth factors in the nucleus have been previously described, but their mechanism of action and biological roles remain enigmatic. Platelet-derived growth factor B (PDGFB) can drive formation of low-grade glioma and here we show that it localizes to the nucleus of human glioma cells where it binds chromatin to preserve genome stability and cell lineage. Failure of PDGFB to localize to the nucleus leads to chromosomal abnormalities, aberrant heterochromatin architecture and accelerated tumorigenesis. Furthermore, nuclear localization of PDGFB is reliant upon the expression levels and mutation status of isocitrate dehydrogenase (IDH). Unexpectedly, we identified macrophages as the predominant source of PDGFB in human, finding that immune-derived PDGFB can localize to the nucleus of glioma cells. Collectively, these studies show that immune derived PDGFB enters the nucleus of glioma cells to maintain genomic stability, while identifying a new mechanism by which IDH mutations promote gliomagenesis.

2024-11-10 | GSE280688 | GEO

Mutant IDH impairs chromatin binding by PDGFB to promote chromosome instability [scATAC-Seq]

2024-11-10 | GSE280597 | GEO

Gene expression profiling and genome-wide maps in PDGFB-induced glioma

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-05-17 | GSE80089 | GEO

Mus musculus

Project description:Genome-wide maps in PDGFB-induced glioma

| PRJNA317783 | ENA

Expression data from RCAS PDGFB/Nestin tv-A glioma cells within Ptprd+/+p16-/- (n=2), Ptprd+/-p16-/- (n=2), and Ptrpd-/-p16-/- (n=3) mice.

Project description:PTPRD is a tumor suppressor of glioma that is frequently co-deleted with CDKN2A/p16. We show that Ptprd and p16 cooperate to promote gliomagenesis in the RCAS PDGFB / Nestin tv-A glioma mouse model. We found unique gene expression changes within tumor cells of Ptprd+/-p16-/- vs. Ptprd+/+p16-/- and Ptprd-/-p16-/- tumor cells. Neonatal mice were injected with RCAS PDGFB GFP. At symptoms, or at 12 weeks post injection, GFP+DAPI- tumor cells were sorted for RNA extraction and hybridization on Affymetrix microarrays. The mouse strain used was a mixed background of C57/BL6 and FVB/N. Ptprd mice were from Uetani et al. 2000 and p16/Nestin-tvA mice were from Tchouganouva et al. 2007.

2014-06-02 | E-GEOD-55061 | biostudies-arrayexpress

Gene expression profiling and genome-wide maps in PDGFB-induced glioma

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-05-17 | E-GEOD-80089 | biostudies-arrayexpress