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ABSTRACT: Analysis of WRKY33 binding sites and WRKY33-dependent gene expression in Arabidopsis thaliana upon Botrytis cinerea 2100 inoculation

OTHER RELATED OMICS DATASETS IN: E-GEOD-66290

PROVIDER: PRJNA276454 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1818390.fastq.gz	Fastqsanger.gz
	SRR1818400.fastq.gz	Fastqsanger.gz
	SRR1818410.fastq.gz	Fastqsanger.gz
	SRR1818391.fastq.gz	Fastqsanger.gz
	SRR1818401.fastq.gz	Fastqsanger.gz

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Similar Datasets

WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. We identified by ChIP-seq >1680 high-confidence WRKY33 binding sites associated with 1576 genes within the Arabidopsis genome, with all of them being dependent on rapid activation of WRKY33 expression by Botrytis cinerea strain 2100. Genome-wide transcriptional analysis defined 318 genes as direct functional targets at 14 h post inoculation. Comparison between resistant wild-type Columbia-0 and susceptible wrky33 mutant plants revealed that expression of 75% of all WRKY33 regulated targets were down-regulated upon infection, indicating that WRKY33 predominately acts as a repressor. However, WRKY33 appears to possess dual functionality acting either as a repressor or as an activator in a promoter-context dependent manner. Our genome-wide analysis confirmed known WRKY33 targets involved in ethylene and jasmonic acid hormone signaling and phytoalexin biosynthesis, but also uncovered a previously unknown role of abscisic acid (ABA) biosynthesis in the complex regulatory circuitry affecting resistance towards Botrytis. Analysis of transgenic plants expressing WRKY33-HA under its native promoter post inoculation with spores of Botrytis cinerea 2100

2015-06-15 | E-GEOD-66289 | biostudies-arrayexpress

WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

Project description:The Arabidopsis thaliana mutant wrky33 is highly susceptible to the necrotrophic fungus Botrytis cinerea. Comparing the expression profiles of B. cinerea-infected wrky33 and WT plants we identified 2765 differentially expressed genes dependent on WRKY33, of which 1675 were up-regulated in the mutant (termed WRKY33-repressed genes) and 1090 were down-regulated in the mutant. Combined with ChIP-seq data 318 genes were identified as direct functional targets of WRKY33 at 14 h post inoculation with spores of Botrytis cinerea 2100. Comparison of altered gene expression in Arabidopsis WT and wrky33 mutant plants 14 hours post inoculation with Botrytis cinerea 2100.

2015-06-15 | E-GEOD-66290 | biostudies-arrayexpress

WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

2015-06-15 | GSE66290 | GEO

WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

2015-06-15 | GSE66289 | GEO

Arabidopsis thaliana

Project description:WRKY33 binding sites in Arabidopsis upon Botrytis cinerea 2100 inoculation

| PRJNA276445 | ENA

Arabidopsis thaliana

Project description:WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

| PRJNA276444 | ENA

E-GEOD-66290

Project description:WRKY33-dependent expression of Arabidopsis genes upon Botrytis cinerea 2100 inoculation

2019-05-14 | E-GEOD-66290 | ExpressionAtlas

Microarray analysis of elp2 and wild type (Col-0) infected with the necrotrophic fungal pathogen Botrytis cinerea

Project description:The goal of the microarray experiment was to identify defense genes that were differentially expressed in the Arabidopsis mutant elp2 and wild type in response to infection of the necrotrophic fungal pathogen Botrytis cinerea. Results indicated that, compared with the wild type, the WRKY33/OCTADECANOID-RESPONSIVE ARABIDOPSIS AP2/ERF59 (ORA59)/ETHYLENE RESPONSE FACTOR1 transcriptional cascades are down-regulated, whereas the MYC2 transcriptional cascade is up-regulated in the elp2 mutant. Three biological replicates with leaves from 8-12 plants per sample were collected at 0, 3, 6, 12, 24, and 48 hours after inoculation with the necrotrophic fungal pathogen Botrytis cinerea. After extraction, RNA concentration was determined on a NanoDrop Spectrophotometer (Thermofisher Scientific, Waltham, MA) and sample quality was assessed using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Equal amount of RNA from the 3 biological replicates were used for microarray analysis. The channels of the dual-channel arrays were analyzed independently.

2013-07-01 | E-GEOD-48207 | biostudies-arrayexpress

Next generation sequencing analysis of Arabidopsis thaliana transcriptomes in response to Botrytis cinerea infection

Project description:Next generation sequencing was performed to identify genes changed in Arabidopsis thaliana upon Botrytis cinerea infection. The goal of the work is to find interesting genes involved in plant defense. The object is to reveal the molecular mechanism of plant defense.

2022-07-20 | GSE186842 | GEO

Analysis of the function of the Arabidopsis DRL1 gene in Botrytis cinerea-induced transcriptome changes

Project description:To define how DRL1 regulates Botrytis cinerea-induced transcriptional changes at the genome level, we performed a microarray experiment on B. cinerea-infected drl1 and wild-type plants to identify genes that were differentially expressed between drl1 and the wild type. A total of 200 defense response genes were differentially expressed between drl1 and the wild type at six hours post-inoculation. These genes include 20 ethylene (ET) responsive genes, 23 jasmonic acid (JA)/ET responsive genes, 50 JA responsive genes, and 107 other defense genes. All ET and JA/ET responsive genes and majorities of JA responsive and other defense genes were down-regulated in the drl1 mutant. Among the down-regulated genes are a group of well-characterized defense genes, including ORA59, ERF1, WRKY33, SIB1, PAD3, GLIP1, and CYP79B2, which have been demonstrated to function in resistance to necrotrophic fungal pathogens, indicating that DLR1 may contribute to B. cinerea resistance by modulating the expression of these defense genes.

2019-09-23 | GSE79961 | GEO

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