Project description:To gain insight into how AtCAPE1 regulates salt response, we investigated the gene expression profiles of wild-type (Ler) and proatcape1 mutant seedlings in the presence and absence of 125 mM NaCl by microarray analysis.
Project description:To gain insight into how AtCAPE1 regulates salt response, we investigated the gene expression profiles of wild-type (Ler) and proatcape1 mutant seedlings in the presence and absence of 125 mM NaCl by microarray analysis. Ten seedlings were grown vertically on the mesh attached on 1/2 MS medium for 10 days. Seedlings with the mesh were transferred to a new petri dish and then covered by buffer-saturated filter papers with 1/2 MS liquid medium (Control) or with 125 mM NaCl (Salt). Salt-treated seedlings (n=10 for each treatment) were sampled after 12 h. Three independent experiments were performed for the microarray analysis.
Project description:The Fusarium incarnatum strain K23, originally isolated from a habit-adapted wild plant Thapsia species, colonized the roots and shoots of tomato seedlings and protected them against salt stress. Comparison of expression and metabolite profile changes uncovered that the fungus completely reprogramed the tomato response to salt stress. Barely any overlap was observed among the genes and metabolites which are regulated by salt stress in uncolonized and colonized tomato seedlings. In colonized seedlings exposed to salt stress, less stress- related genes are activated than in un-colonized seedlings. Furthermore, K23 produced gibberellin and gibberellin-responsive genes were detected in all RNA samples. Our analysis demonstrates that K23 colonisation completely alters the salt-responsive gene and metabolite profiles in tomato seedlings.
Project description:To further investigate the gene expression profiles in agb1 mutant seedlings responding to salt stress, we employed whole transcriptome microarray expression profiling as a discovery platform to identify genes with the potential to be regulated by AGB1-mediated signaling cascades. Arabidopsis thaliana 7-day-old wild-type and agb1-3 mutant roots were challenged by immersing in 150 mM NaCl containing MS liquid media for 0 or 4 hours, and 4,830 genes induced by high salinity were identified that distinguished between zero and four hours among wild-type and agb1-3 samples. Thirteen of the 22 gene expressions that are transcriptionally regulated by bZIP17 under salt stress were decreased in agb1-3 compared to wild-type roots. The expression of three genes (NAC019, RD20, and TSPO) from this group was quantified in the same RNA samples by real-time PCR, confirming lower expression in both agb1-3 and bzip17-4 roots.
Project description:Transcriptional profiling of Arabidopsis thaliana Ler wildtype and eid3 (empfindlicher im dunkelroten Licht 3) mutant seedlings in darkness and 45 min after a red-light pulse.
Project description:gen45-carpel_development - crc1 mutant - Identification of target genes from three transcription factors involved in gynoecium development - Comparison between the wild type Ler and crc1 mutant. Keywords: gene knock out
Project description:Accumulation of unfolded/misfolded proteins in endoplasmic reticulum (ER) elicits a well conserved response called the Unfolded Protein Response (UPR), which triggers the up-regulation of downstream genes involved in protein folding, vesicle trafficking, and ER-Associated Degradation (ERAD). Although the dynamic transcriptomic responses and underlying major transcriptional regulators in ER stress response in plants have been well established, the proteome changes induced by ER stress have not been reported in plants. In the current study, we found that the Arabidopsis Ler ecotype is more sensitive to ER stress than the Col ecotype. Quantitative mass spectrometry analysis with Tandem Mass Tag (TMT) isobaric labeling showed that totally 7439 and 7035 proteins were identified from Col and Ler seedlings, with 88 and 113 differentially regulated (FC>1.3 or <0.7, P<0.05) proteins by ER stress in Col and Ler, respectively. Among them, 40 proteins were commonly up-regulated in Col and Ler, of which 10 were not up-regulated in bzip28 bzip60 double mutant (Col background) plants. Of the 19 specifically up-regulated proteins in Col comparing to that in Ler, components in ERAD, N-glycosylation, vesicle trafficking and molecular chaperones were represented. Quantitative RT-PCR showed that genes encoding 7 out of 19 proteins were not up-regulated (FC>1.3 or <0.7, P<0.05) by ER stress in both ecotypes while genes encoding 12 out of 19 proteins were up-regulated by ER stress with no obvious differences in fold change between Col and Ler. Our results experimentally demonstrated the robust ER stress response at proteome level in plants and revealed differentially regulated proteins that may contribute to differed ER stress sensitivity between Col and Ler ecotypes in Arabidopsis.
Project description:High salinity is one of the major environmental factors, which hampers plant growth, development and productivity. To better understand the regulatory mechanisms by which plants cope with salt stress, we used genetic approaches to identify salt hypersensitive mutant 9 (sahy9), a new allele of apum23, in Arabidopsis thaliana. The sahy9/apum23 mutant seedlings display postgemination developmental arrest and later become bleached under agar plates supplemented with various salt stressors. Transcriptomic and proteomic analyses of the salt-treated sahy9/apum23 and wild-type seedlings revealed differential expression of genes with similar functional categories, primarily including cellular and metabolic processes, and abiotic and biotic stress responses. However, the consistency of gene expression at both transcript and protein levels is low (), suggesting the involvement of posttranscriptional processing in salt response. Furthermore, the altered gene/protein expression mediated by SAHY9/APUM23 in salt sensitivity is involved in several functional groups, particularly in ABA biosynthesis and signaling, abiotic stress response, LEA proteins, and ribosome biogenesis-related genes. Importantly, NCED3, a key gene involved in ABA biosynthesis, and major ABA responsive marker genes, such as RD20 and RD29B, are down-regulated at both transcript and protein levels in sahy9/apum23 under salt stress. Consistently, lower contents of ABA and proline, and expression changes of a subset of LEA proteins also support the nature of sahy9/apum23 showing salt hypersensitivity. Collectively, these data suggest that SAHY9/APUM23-mediated salt response is associated with ABA signaling pathway and its downstream stress responsive or tolerant genes.
