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Bos taurus

ABSTRACT: Identification of novel, highly expressed retroviral microRNAs in cells infected by bovine foamy virus

PROVIDER: PRJNA280497 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1955231.fastq.gz	Fastqsanger.gz
	SRR1955232.fastq.gz	Fastqsanger.gz
	SRR1955233_1.fastq.gz	Fastqsanger.gz
	SRR1955233_2.fastq.gz	Fastqsanger.gz

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Identification of novel, highly expressed retroviral microRNAs in cells infected by bovine foamy virus

Project description:While numerous viral microRNAs (miRNAs) expressed by DNA viruses, especially herpesvirus family members, have been reported, there have been very few reports of miRNAs derived from RNA viruses. Here we describe three miRNAs expressed by bovine foamy virus (BFV), a member of the spumavirus subfamily of retroviruses, in both BFV-infected cultured cells and BFV-infected cattle. All three viral miRNAs are initially expressed in the form of an ∼ 122-nucleotide (nt) pri-miRNA, encoded within the BFV long terminal repeat U3 region, that is subsequently cleaved to generate two pre-miRNAs that are then processed to yield three distinct, biologically active miRNAs. The BFV pri-miRNA is transcribed by RNA polymerase III, and the three resultant mature miRNAs were found to contribute a remarkable ∼ 70% of all miRNAs expressed in BFV-infected cells. These data document the second example of a retrovirus that is able to express viral miRNAs by using embedded proviral RNA polymerase III promoters. Small RNA profiling of Bovine Foamy Virus (BFV)-infected cells through total small RNA deep sequencing

2015-04-07 | E-GEOD-67620 | biostudies-arrayexpress

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