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Dual RNA-seq – Pulse-expression of pinT

ABSTRACT: Dual RNA-seq – Pulse-expression of pinT

OTHER RELATED OMICS DATASETS IN: GSE67950GSE67759GSE67951GSE67757GSE67948GSE67952GSE67758

PROVIDER: PRJNA281340 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1976380.fastq.gz	Fastqsanger.gz
	SRR1976390.fastq.gz	Fastqsanger.gz
	SRR1976381.fastq.gz	Fastqsanger.gz
	SRR1976382.fastq.gz	Fastqsanger.gz
	SRR1976383.fastq.gz	Fastqsanger.gz

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Dual RNA-seq – Host response to pinT*

Project description:Dual RNA-seq – Host response to pinT*

| PRJNA281339 | ENA

Dual RNA-seq – Pulse-expression of pinT

Project description:Bacteria possess many small noncoding RNAs whose regulatory roles in pathogenesis are little understood due to a paucity of macroscopic phenotypes in standard virulence assays. Here, we use a novel Dual RNA-seq approach for a single-step simultaneous RNA profiling in both pathogen and host to reveal molecular phenotypes of sRNAs during infection with Salmonella Typhimurium. We identify a new PhoP/Q-activated small RNA which upon bacterial internalization acts to temporally control the expression of both, invasion-associated effectors and virulence genes required for intracellular survival. This riboregulatory activity is shown to adjust the human response to replicating Salmonella, and have a pervasive impact on host RNA expression both inside and outside protein-coding regions including infection-specific alterations of an array of long noncoding RNAs. Our study provides a paradigm for a comprehensive RNA-based analysis of intracellular bacterial pathogens without their physical purification from a host and a new discovery route for hidden functions of pathogen genes.

2015-12-11 | GSE67950 | GEO

PhrS pulse expression RNA-seq

Project description:PhrS pulse expression RNA-seq

| PRJNA966772 | ENA

TWIST1 methylation by SETD6 selectively antagonizes LINC-PINT expression in Glioblastoma multiforme

Project description:Glioblastoma multiforme (GBM) is the most common and aggressive malignant brain tumor among adults, which is characterized by high invasion, migration and proliferation abilities. One important process that contributes to the invasiveness of GBM is the epithelial to mesenchymal transition (EMT). EMT is regulated by a set of defined transcription factors which tightly regulate this process, among them is the basic helix-loop-helix family member, TWIST1. Here we show that TWIST1 is methylated on lysine-33 at chromatin by SETD6, a methyltransferase with expression levels correlating with poor survival in GBM patients. RNA-seq analysis in U251 GBM cells suggested that both SETD6 and TWIST1 regulate cell adhesion and migration processes. We further show that TWIST1 methylation attenuates the expression of the long-non-coding RNA, LINC-PINT, thereby suppressing EMT in GBM. Mechanistically, TWIST1 methylation represses the transcription of LINC-PINT by increasing the occupancy of EZH2 and the catalysis of the repressive H3K27me3 mark at the LINC-PINT locus. Under un-methylated conditions, TWIST1 dissociates from the LINC-PINT locus, allowing the expression of LINC-PINT which leads to increased cell adhesion and decreased cell migration. Together, our findings unravel a new mechanistic dimension for selective expression of LINC-PINT mediated by TWIST1 methylation.

2022-08-12 | PXD029361 | Pride

Dual RNA-seq – Pilot Dual RNA-seq

Project description:Dual RNA-seq – Pilot Dual RNA-seq

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Dual RNA-seq – Host response to pinT*

2015-12-11 | GSE67951 | GEO

An NF-κB-responsive long noncoding RNA, PINT, regulates inflammatory gene transcription by scaffolding p65 and EZH2

Project description:Long non-coding RNAs (lncRNAs) are central regulators of the inflammatory response and play an important role in inflammatory diseases. PINT has been reported to be involved in tissue development and tumorigenesis. However, potential functions of PINT in the innate immune system are largely unknown. Here, we report a specific role for PINT in activating inflammatory gene transcription. The expression of PINT is primarily dependent on the NF-κB signaling pathway in macrophages and intestinal epithelial cells. Functionally, PINT selectively promotes the expression of inflammatory genes, including TNF-α, in basal and LPS-stimulated cells. Mechanistically, PINT acts as a modular scaffold of p65 and EZH2 to coordinate their localization and specify their binding to the target genes. Further, a high expression of PINT is detected in the intestinal tissue from patients with Inflammatory Bowel Disease (IBD). Together, these findings demonstrate that PINT acts as an activator of inflammatory responses, highlighting the importance of this lncRNA in inflammatory diseases as a potential therapeutic target.

2022-10-01 | GSE139278 | GEO

Dual RNA-seq – Dual RNA-seq of further sRNA mutants

Project description:Dual RNA-seq – Dual RNA-seq of further sRNA mutants

| PRJNA281338 | ENA

Dual RNA-seq – Comparative Dual RNA-seq in pig macrophages

Project description:Dual RNA-seq – Comparative Dual RNA-seq in pig macrophages

| PRJNA281342 | ENA

Dual RNA-seq – High-resolution comparative Dual RNA-seq time-course

Project description:Dual RNA-seq – High-resolution comparative Dual RNA-seq time-course

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