Dual RNA-seq – Pulse-expression of pinT

ABSTRACT: Bacteria possess many small noncoding RNAs whose regulatory roles in pathogenesis are little understood due to a paucity of macroscopic phenotypes in standard virulence assays. Here, we use a novel Dual RNA-seq approach for a single-step simultaneous RNA profiling in both pathogen and host to reveal molecular phenotypes of sRNAs during infection with Salmonella Typhimurium. We identify a new PhoP/Q-activated small RNA which upon bacterial internalization acts to temporally control the expression of both, invasion-associated effectors and virulence genes required for intracellular survival. This riboregulatory activity is shown to adjust the human response to replicating Salmonella, and have a pervasive impact on host RNA expression both inside and outside protein-coding regions including infection-specific alterations of an array of long noncoding RNAs. Our study provides a paradigm for a comprehensive RNA-based analysis of intracellular bacterial pathogens without their physical purification from a host and a new discovery route for hidden functions of pathogen genes.

OTHER RELATED OMICS DATASETS IN: PRJNA280839 PRJNA281342 PRJNA281339 PRJNA281343 PRJNA280838 PRJNA281341 PRJNA280840 PRJNA281340 PRJNA281338

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 Homo sapiens

PROVIDER: GSE67950 | GEO | 2015/12/11

SECONDARY ACCESSION(S): PRJNA281340

REPOSITORIES: GEO