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TFIID subunit TAF4 is essential at mid-gestation of murine embryogenesis and for embryonic stem cell differentiation

ABSTRACT: TFIID subunit TAF4 is essential at mid-gestation of murine embryogenesis and for embryonic stem cell differentiation

PROVIDER: PRJNA289445 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2096290.fastq.gz	Fastqsanger.gz
	SRR2096300.fastq.gz	Fastqsanger.gz
	SRR2096261.fastq.gz	Fastqsanger.gz
	SRR2096291.fastq.gz	Fastqsanger.gz
	SRR2096301.fastq.gz	Fastqsanger.gz

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A TAF4 coactivator function for E proteins that involves enhanced TFIID binding

Project description:The multisubunit TFIID plays a direct role in transcription initiation by binding to core promoter elements and directing preinitiation complex assembly. Although TFIID may also function as a coactivator through direct interactions with promoter-bound activators, mechanistic aspects of this poorly defined function remain unclear. Here biochemical studies show a direct TFIID-E protein interaction that (i) is mediated through interaction of a novel E protein activation domain (AD3) with the TAF homology (TAFH) domain of TAF4, (ii) is critical for activation of a natural target gene by an E protein and (iii) mechanistically, acts by enhancing TFIID binding to the core promoter. Complementary assays establish a gene-specific role for the TAFH domain in TFIID recruitment and gene activation in vivo. These results firmly establish TAF4 as a bona fide E protein coactivator, as well as a mechanism involving facilitated TFIID binding through direct interaction with an E protein activation domain. Genome-wide profiling of mRNA levels in MEF lines with Taf4 loxp/- (ctrl), Taf4 -/- (ko), Taf4 -/- Tg:hTAF4 wt, and Taf4 -/- Tg: hTAF4 ? (TAFH-deleted).

2013-06-06 | E-GEOD-46807 | biostudies-arrayexpress

The TFIID subunit TAF4 is required for pancreatic beta cell function and identity [RNA-seq]

Project description:We selectively inactivated the Taf4 subunit of general transcription factor TFIID in adult murine pancreatic beta cells (BCs). Taf4 inactivation rapidly diminishes expression of critical genes involved in BC function leading to increased glycaemia, lowered blood insulin levels, defective glucose-stimulated insulin secretion and in the longer term reduced BC mass through apoptosis of a subpopulation of BCs. Nevertheless, glycaemia and blood insulin levels are stabilised after 11 weeks with mutant animals showing long term survival. Bulk RNA-seq and ATAC-seq and single cell RNA-seq on isolated Langerhans islets show that Taf4 loss leads to major remodelling of chromatin accessibility and gene expression not only in targeted BCs, but also alpha and delta cells. One week after Taf4-loss cells with mixed BC, alpha and/or delta cell identities were observed as well as a population trans-differentiating into alpha cells. Trans-differentiation was associated with the concerted action of the Mafb, Mafg, Bptf and Foxp1 transcription factors. Taf4 is therefore essential for BC function and identity and we identify a novel set of factors associated with BC trans-differentiation.

2021-06-01 | GSE151364 | GEO

The TFIID subunit TAF4 is required for pancreatic beta cell function and identity [ATAC-seq]

2021-06-01 | GSE151363 | GEO

The TFIID subunit TAF4 is required for pancreatic beta cell function and identity [single-cell RNA-seq]

2021-06-01 | GSE151365 | GEO

A TAF4 coactivator function for E proteins that involves enhanced TFIID binding

2013-06-06 | GSE46807 | GEO

TFIID subunit TAF4 is essential at mid-gestation of murine embryogenesis and for embryonic stem cell differentiation

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-03-20 | GSE70726 | GEO

Essential role of the TFIID subunit TAF4 in murine embryogenesis and embryonic stem cell differentiation.

Project description:TAF4 (TATA-binding protein-associated factor 4) and its paralogue TAF4b are components of the TFIID core module. We inactivated the murine Taf4a gene to address Taf4 function during embryogenesis. Here we show that Taf4a(-/-) embryos survive until E9.5 where primary germ layers and many embryonic structures are identified showing Taf4 is dispensable for their specification. In contrast, Taf4 is required for correct patterning of the trunk and anterior structures, ventral morphogenesis and proper heart positioning. Overlapping expression of Taf4a and Taf4b during embryogenesis suggests their redundancy at early stages. In agreement with this, Taf4a(-/-) embryonic stem cells (ESCs) are viable and comprise Taf4b-containing TFIID. Nevertheless, Taf4a(-/-) ESCs do not complete differentiation into glutamatergic neurons and cardiomyocytes in vitro due to impaired preinitiation complex formation at the promoters of critical differentiation genes. We define an essential role of a core TFIID TAF in differentiation events during mammalian embryogenesis.

| S-EPMC4820908 | biostudies-literature

The TFIID subunit TAF4 is required for pancreatic beta cell function and identity

Project description:The TFIID subunit TAF4 is required for pancreatic beta cell function and identity

| PRJNA635627 | ENA

Gene Expression of mid-gestation human placenta

Project description:Gene Expression of mid-gestation human placenta

| PRJNA737475 | ENA

Gene Expression of mid-gestation human placenta

Project description:The goal of this study was to transciprtionally profile the three layers of the human placenta (decidua, fetal membrane and placental villi) from the mid-gestation healthy human placenta.

2022-01-27 | GSE178152 | GEO

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