Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA295014 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR2295920.fastq.gz | Fastqsanger.gz | |||
| SRR2295921.fastq.gz | Fastqsanger.gz | |||
| SRR2295922.fastq.gz | Fastqsanger.gz | |||
| SRR2295923.fastq.gz | Fastqsanger.gz | |||
| SRR2295924.fastq.gz | Fastqsanger.gz |
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2016-07-28 | E-GEOD-72762 | biostudies-arrayexpress
Project description:Aim: Transcriptional analysis of the duodenum of adult Nkx2.2flox/SD;Villin-Cre (SDint) mice versus control Methods: 2 cm of the duodenum (as measured from the stomach) of 6 week old control and mutant mice were dissected and total RNA extracted. Libraries were prepared from total RNA (RIN>8) with the TruSeq RNA prep kit (Illumina) and sequenced using the HiSeq2000 (Illumina) instrument. More than 20 million reads were mapped to the mouse genome (UCSC/mm9) using Tophat (version 2.0.4) with 4 mismatches and 10 maximum multiple hits. Significantly differentially expressed genes were calculated using DEseq. Results: 206 genes with a p-value <0.05 were significantly changed. Among these are some enteroendocrine hormones. Conclusion: The SD domain of Nkx2.2 regulates specification of some enteroendocrine cells
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