ABSTRACT: Study of the genome and virulence of Haemophilus influenzae biotype aegyptius strains associated with Brazilian Purpuric Fever - strain F3042
Project description:Study of the genome and virulence of Haemophilus influenzae biotype aegyptius strains associated with Brazilian Purpuric Fever - strain KC1018
Project description:Study of the genome and virulence of Haemophilus influenzae biotype aegyptius strains associated with Brazilian Purpuric Fever - strain F3030
Project description:Study of the genome and virulence of Haemophilus influenzae biotype aegyptius strains associated with Brazilian Purpuric Fever - strain F3039
Project description:Study of the genome and virulence of Haemophilus influenzae biotype aegyptius strains associated with Brazilian Purpuric Fever - strain F1946
Project description:Study of the genome and virulence of Haemophilus influenzae biotype aegyptius strains associated with Brazilian Purpuric Fever - strain F3283
Project description:Cytoplasmic DNA methyltransferases are often associated with restriction modification (R-M) systems in bacterial systems. Phase variable expression of these DNA methyltransferase results in epigenetic regulation of multiple genes as part of systems called phasevarions (phase-variable regulons). H. aegyptius is the causative agent of Brazilian Purpuric Fever (BPF), an invasive disease with high mortality, that sporadically manifests in children previously suffering conjunctivitis. We have recently described a previously unidentified allele of a phase variable Type III DNA methylstransferase in H. aegyptius, ModA16. When ModA16 is expressed, H. aegyptius strains exhibit differential methylation throughout the genome compared to when ModA16 is not expressed. This change in methylation results in altered gene expression and SWATH-MS was used to identify the resulting changes to protein expression. This dataset represents triplicate repeats from strains which express ModA16 (ON) and strains which do not (OFF).
Project description:The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the las transfer to search an elucidation of the pathogenic factors by horizontal intergeneric transfer from meningococci to H. influenzae.