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Mus musculus

ABSTRACT: Genome-wide mapping of PRDM15 binding in pluripotent mouse Embryonic Stem Cells (mESCs)

PROVIDER: PRJNA297676 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2550342.fastq.gz	Fastqsanger.gz
	SRR2550343.fastq.gz	Fastqsanger.gz
	SRR2550334.fastq.gz	Fastqsanger.gz
	SRR2550344.fastq.gz	Fastqsanger.gz
	SRR2550335.fastq.gz	Fastqsanger.gz

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Genome-wide mapping of PRDM15 binding in pluripotent mouse Embryonic Stem Cells (mESCs)

Project description:Genome-wide mapping of PRDM15 binding in pluripotent mouse Embryonic Stem Cells (mESCs)

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Role of Prdm14 in mouse embryonic stem cells: ChIP-seq and RNA-seq analyses

Project description:Prdm14 is a PR-domain and zinc-finger protein whose expression is restricted to the pluripotent cells of an early embryo, embryonic stem cells (ESCs), and germ cells. Here we show that Prdm14 safeguards mouse ESC maintenance by preventing induction of extraembryonic endoderm (ExEn) fates. Conversely, Prdm14 overexpression impairs ExEn differentiation during embryoid body (EB) formation. Prdm14 occupies and represses genomic loci encoding ExEn differentiation factors, while also binding to and promoting expression of genes associated with ESC self-renewal. Prdm14-bound genomic regions significantly overlap those occupied by Nanog and Oct4, are enriched in a chromatin signature associated with distal regulatory elements, and contain a unique DNA-sequence motif recognized by Prdm14 in vitro. Our work identifies Prdm14 as a new member of mouse ESC (mESC) transcriptional network, which plays a dual role as a context-dependent transcriptional repressor or activator at distal silencers and enhancers. [ChIP-seq] Genome-wide mapping of Prdm14 binding sites in mouse embryonic stem cells: A FLAG-HA tagged Prdm14 (FH-Prdm14) mESC line was established. FLAG-HA double ChIP (ChIP with FLAG antibody followed by ChIP with HA antibody) was performed with FH-Prdm14 mESCs (Prdm14-ChIPseq) and as a negative control, wildtype mESCs (FLAG-HA_ChIPseq). H3K4me1 ChIPseq in mouse ES cells. Using published H3K4me1 data, we found there is a correlation between Prdm14 binding and H3K4me1 marks. So we obtained our own H3K4me1 data, using the wildtype mESCs. [RNA-seq] Global RNAseq analysis of Prdm14 knockdown in mouse embryonic stem cells: Analysis of poly(A)+ RNA from mESCs treated with non-targeting control siRNA and Prdm14 siRNA.

2010-12-23 | E-GEOD-25409 | biostudies-arrayexpress

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Project description:Expression data from induced pluripotent stem cells (iPSCs), mouse embryonic stem cells (mESCs), and mouse embryonic fibroblast (MEFs)

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Novel O-GlcNAcylation on Thr71 of histone H4 preferentially localizes to active gene regions

Project description:We report genome-wide distribution of newly discovered O-GlcNAcylated H4 at threonine 71 (H4T71Gc) in mouse embryonic stem cells (mESCs, J1 line) cultured in 25 mM glucose (mESCs-25mMG) or 1 mM glucose (mESCs-1mMG) condition, human embryonic stem cells (hESCs, H9) and human induced-pluripotent stem cells (hiPSCs, 201B7). We found that H4T71Gc preferentially localize to gene regions, especially those with relatively high expression levels. Interestingly, the localized gene region of H4T71Gc changes in response to changes in extracellular glucose concentration. This study using ChIP-seq analysis provides genomic distribution of novel histone O-GlcNAcylation in mouse and human pluripotent stem cells.

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Project description:Genome-wide mapping of Klf4 and Klf5 in pluripotent mouse embryonic stem cells (ESCs)

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Kdm2b maintains murine embryonic stem cell status by recruiting PRC1 complex to CpG islands of lineage genes [Expression profiling]

Project description:Polycomb group (PcG) proteins play important roles in repressing lineage-specific genes and maintaining the undifferentiated state of mouse embryonic stem cells (mESCs). However, the mechanisms by which PcG proteins are recruited to their targets are largely unknown. Here, we show that the histone demethylase Kdm2b is highly expressed in mESCs and regulated by the pluripotent factors Oct4/Sox2 directly. Depletion of Kdm2b in mESCs causes de-repression of lineage-specific genes and induces early differentiation. The function of Kdm2b depends on its CXXC-ZF domain, which mediates Kdm2b’s genome-wide binding to CpG islands (CGIs). Kdm2b interacts with the core components of the Polycomb repressive complex 1 (PRC1) and recruits the complex to the CGIs of early lineage-specific genes. Thus, our study not only reveals a novel Oct4/Sox2-Kdm2b-PRC1-CGI regulatory axis and its function in maintaining undifferentiated state of mESCs, but also demonstrates a critical function of Kdm2b in recruiting PRC1 to the CGIs of lineage-specific genes to repress their expression. In this dataset, we include the expression data for control and Kdm2b knockdown mouse embryonic stem cells. We analyzed the gene expression in control and Kdm2b knockdown mouse embryonic stem cells using the Affymetrix MoGene-1_0-st-v1 platform.

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Genomic context sensitizes regulatory elements to genetic disruption [RNA]

Project description:DNase-seq mapping in engineered mesendodermal cells differentiated from mouse embryonic stem cells (mESCs) using CHIR.

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Glycine Cleavage System Determines the Fate of Pluripotent Stem Cells via the Regulation of Senescence and Epigenetic Modifications

Project description:To investigate whether embryonic stem cells exhibit a metabolic state different from that of somatic cells, we performed RNA-seq in mouse embryonic fibroblasts (MEFs), induced pluripotent stem cells (iPSCs) and mouse embryonic stem cells (mESCs). To further investigate the effect of Gldc in mESCs, we performed RNA-seq in V6.5 cells with Gldc knockdown.

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