Project description:Rathayibacter toxicus is a gram-positive bacterium that is the causative agent of annual ryegrass toxicity, a disease that causes devastating losses in the Australian livestock industry. This bacterium is poorly characterized, making it difficult to accurately detect in feed samples. Using 1-D gels and mass spectrometry, we analyzed the protein expression of R. toxicus under stationary growth phase conditions to obtain a more complete understanding of the mechanisms of this organism. A total of 333 unique proteins were identified. The data obtained in this analysis is an essential first step toward developing an antibody-based diagnostic assay.
