Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA298561 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of E14 Dlk+ cells derived from Matrix metalloproteinase (MMP)-14 deficient (KO) mice comparing those from littermate wild-type (WT) mice. RNA samples were extrated from FACS-sorted Dlk+CD45-CD71-Ter119- cells derived from E14.5 livers. Transcriptional profiling of postnatal day (P)1 livers derived from MMP-14 deficient (KO) mice comparing those from littermate wild-type (WT) mice. RNA samples were extrated from whole livers derived from P1 mice.
2015-12-25 | GSE73943 | GEO
Project description:LPS/GalN-induced liver injury model: wild-type vs. TMX-deficient mice
| PRJNA139245 | ENA
Project description:Heme Deficient vs Wild Type in Low Iron
| PRJNA93417 | ENA
Project description:The homeobox transcription factor Prox1 is expressed in embryonic hepatoblasts and remains expressed in adult hepatocytes. Prox1-null mice show severe deficiencies of liver development, but the underlying mechanisms are unknown. We studied the effects of Prox1 on the transcriptional profile of embryonic day-14 (ED14) met-murine-hepatocytes (ED14-MMH). These immortalized murine hepatoblasts express numerous hepatoblast markers, but not Prox1. We performed stable transfection with Prox1 cDNA, analyzed the transcriptome with Agilent mouse whole genome microarrays and validated genes by qRT-PCR. We observed more than 12-fold up-regulation of 22 genes and down-regulation of 232 genes. Numerous of these genes are involved in metabolic hepatocyte functions and may be regulated by Prox1 directly or indirectly, e.g. by down-regulation of HNF4a. Prox1 induces down-regulation of transcription factors, which are highly expressed in neighboring endodermal organs, suggesting a function during hepatoblast commitment. Prox1 does not influence proliferative activity of MMH but regulates genes involved in liver morphogenesis. We observed up-regulation of both type-IVa3 procollagen and functionally active matrix metalloproteinase-2 (MMP-2), which places Prox1 in the centre of liver matrix turnover. This is consistent with MMP-2 expression in hepatoblasts during liver development, and persistence of a basal lamina around the liver bud in Prox1-deficient mice. Our studies suggest that Prox1 is a multifunctional regulator of liver morphogenesis, hepatocyte function and commitment. Keywords: Met murine hepatocytes - Hex - HNF4a - MMP-2 - Type-IV collagen Keywords: Met murine hepatocytes - Hex - HNF4a - MMP-2 - Type-IV collagen
2007-05-25 | GSE7867 | GEO
Project description:There are three projects including AE155LQ, RE161LQ, RE235LQ. AE155LQ is about the proteomes of wild type cells (WT) and E. coli RelA* OE strain during exponential growth in glucose cAA medium. E1 & E2 corresponds to the wild type cells (two biological replicate samples) and E3 & E4 corresponds to RelA* OE data (two biological replicate samples). RE161LQ is about the time-course proteome of wild type (four samples including WT_0, WT_20, WT_40 and WT_80) vs relA-deficient strain (relA_0, relA_1.5 h, relA_3 h and relA_4.5 h) during AA downshift. RE235Q is about the time-course proteome of wild type (three samples including WT_0, WT_60, WT_160) vs relA-deficient strain (three samples including relA_0, relA_60 and relA_160) during carbon downshift.
2023-01-26 | PXD035675 | Pride
Project description:NO-deficient vs wild type Col-0 Arabidopsis seedlings
| PRJNA178593 | ENA
Project description:The homeobox transcription factor Prox1 is expressed in embryonic hepatoblasts and remains expressed in adult hepatocytes. Prox1-null mice show severe deficiencies of liver development, but the underlying mechanisms are unknown. We studied the effects of Prox1 on the transcriptional profile of embryonic day-14 (ED14) met-murine-hepatocytes (ED14-MMH). These immortalized murine hepatoblasts express numerous hepatoblast markers, but not Prox1. We performed stable transfection with Prox1 cDNA, analyzed the transcriptome with Agilent mouse whole genome microarrays and validated genes by qRT-PCR. We observed more than 12-fold up-regulation of 22 genes and down-regulation of 232 genes. Numerous of these genes are involved in metabolic hepatocyte functions and may be regulated by Prox1 directly or indirectly, e.g. by down-regulation of HNF4a. Prox1 induces down-regulation of transcription factors, which are highly expressed in neighboring endodermal organs, suggesting a function during hepatoblast commitment. Prox1 does not influence proliferative activity of MMH but regulates genes involved in liver morphogenesis. We observed up-regulation of both type-IVa3 procollagen and functionally active matrix metalloproteinase-2 (MMP-2), which places Prox1 in the centre of liver matrix turnover. This is consistent with MMP-2 expression in hepatoblasts during liver development, and persistence of a basal lamina around the liver bud in Prox1-deficient mice. Our studies suggest that Prox1 is a multifunctional regulator of liver morphogenesis, hepatocyte function and commitment. Experiment Overall Design: - two samples Experiment Overall Design: - Dye Swap design with 6 arrays
2008-06-15 | E-GEOD-7867 | biostudies-arrayexpress