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GalR Acts as a Transcriptional Activator of galKT in the Presence of Galactose in Streptococcus pneumoniae

ABSTRACT: GalR Acts as a Transcriptional Activator of galKT in the Presence of Galactose in Streptococcus pneumoniae

PROVIDER: PRJNA300565 | ENA |

REPOSITORIES: ENA

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GalR Acts as a Transcriptional Activator of galKT in the Presence of Galactose in Streptococcus pneumoniae

Project description:We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3' ) for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. Growth comparison of Δ galK with the D39 wildtype strain grown in the presence of galactose shows that galK is required for the proper growth of S. pneumoniae on galactose. This SuperSeries is composed of the SubSeries listed below.

2015-10-30 | GSE74502 | GEO

Impact of galR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Galactose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔgalR to D39 wild type grown in M17 medium+ 0.5% (w/v) galactose (GalM17).

2015-10-30 | GSE74501 | GEO

Transcriptional response of Streptococcus pneumoniae D39 to Galactose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium + 0.5 % (w/v) Galactose (GaM17) to grown in M17 medium + 0.5 % (w/v) Glucose (GM17).

2014-06-20 | GSE58180 | GEO

Comparison of the Streptococcus pneumoniae D39 wild-type to the D39 galR mutant [GalM17]

Project description:We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3' ) for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. Growth comparison of Î galK with the D39 wildtype strain grown in the presence of galactose shows that galK is required for the proper growth of S. pneumoniae on galactose. Comparison of the Streptococcus pneumoniae D39 galR mutant compared to D39 wild type in M17 medium+ 0.5% (w/v) galactose (GalM17). One condition design comparison of two strains including a dye swap.

2015-10-30 | E-GEOD-74501 | biostudies-arrayexpress

Comparison of the Streptococcus pneumoniae D39 wild-type to the D39 galR mutant [GM17]

Project description:We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3' ) for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. Growth comparison of Î galK with the D39 wildtype strain grown in the presence of galactose shows that galK is required for the proper growth of S. pneumoniae on galactose. Comparison of the Streptococcus pneumoniae D39 galR mutant compared to D39 wild type in M17 medium+ 0.5% (w/v) glucose (GM17). One condition design comparison of two strains including a dye swap.

2015-10-30 | E-GEOD-74500 | biostudies-arrayexpress

Galactose Vs Glucose

Project description:Comparison of the Streptococcus pneumoniae D39 wild type in M17 medium+ 0.5 % (w/v) Galactose (GaM17) compared to M17 medium+ 0.5 % (w/v) Glucose (GM17) Two condition design comparison of Wild-type strain including a dye swap

2014-06-20 | E-GEOD-58180 | biostudies-arrayexpress

ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Galactose at MID-log growth phase

Project description:Comparison of Streptococcus pneumoniae D39 ccpA mutant compared to D39 wild type in CDM with galactose as sole carbon source to define the regulon of carbon catabolite control protein CcpA under this condition Details described in Carvalho SM, Kloosterman TG, Neves AR, Kuipers OP. CcpA Ensures Optimal Metabolic Fitness of Streptococcus pneumoniae. Plos One 2011

2011-10-01 | GSE31817 | GEO

Neuraminidase-exposed galactose promotes Streptococcus pneumoniae biofilm formation during colonization

Project description:Galactose promotes pneumococcal biofilms in vivo 15 mRNA profiles of Streptococcus pneumoniae samples that were grown under different conditions were generated using deep sequencing.

2016-08-04 | E-GEOD-85196 | biostudies-arrayexpress

ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + galactose at transition-phase of growth (TS)

2011-10-01 | GSE31818 | GEO

The role of the transcription factors LacR and LacT in the regulation of the lactose and galactose utilization pathways in Streptococcus pneumoniae

Project description:Abstract: Transcriptome comparison of the Streptococcus pneumoniae strain D39 grown in the presence of either lactose or galactose with that of the strain grown in the presence of glucose, revealed the elevated expression of various genes and operons, including the lac gene cluster that is organized into two operons i.e. lac operon-I (lacABCD) and lac operon-II (lacTFEG). Deletion of the DeoR family transcriptional regulator lacR that is present downstream of the lac gene cluster, revealed elevated expression of lac operon-I, even in the absence of lactose. This suggests a function of LacR as a transcriptional repressor of lac operon-I that encodes enzymes involved in the Tagatose-6-P pathway in the absence of lactose or galactose. Deletion of lacR did not affect the expression of lac operon-II that encodes for a lactose-specific PTS. This finding was further confirmed by ?-galactosidase assays with PlacA-lacZ and PlacT-lacZ in the presence of either lactose or glucose as a sole carbon source in the medium. This suggests the presence of another transcriptional regulator in the regulation of lac operon-II, which could be the BglG-family transcriptional antiterminator LacT. We demonstrate the role of LacT as a transcriptional activator of lac operon-II in the presence of lactose and CcpA-independent regulation of the lac gene cluster in S. pneumoniae. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-06-20 | E-GEOD-58184 | biostudies-arrayexpress

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