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Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress

ABSTRACT: Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress

OTHER RELATED OMICS DATASETS IN: GSE74820

PROVIDER: PRJNA301598 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1521750.fastq.gz	Fastqsanger.gz
	SRR1521760.fastq.gz	Fastqsanger.gz
	SRR1521770.fastq.gz	Fastqsanger.gz
	SRR1521751.fastq.gz	Fastqsanger.gz
	SRR1521761.fastq.gz	Fastqsanger.gz

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Similar Datasets

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

Project description:Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

| PRJNA255780 | ENA

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

Project description:Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

| PRJNA301602 | ENA

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

Project description:We use RNA-Seq to investigate gene expression differences in response to saltwater exposure by the larval stages of the obligate freshwater mosquito Anopheles coluzzii (formerly An. gambiae "M" form), and the euryhaline An. merus. After rearing in freshwater, both young and old larval instars of each species were briefly (6 h) exposed to either saltwater (SW) or freshwater (FW) conditions to test the impact of salinity on mRNA levels.

2016-03-09 | E-GEOD-59632 | biostudies-arrayexpress

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set1]

2016-03-09 | GSE59632 | GEO

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

Project description:We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity.

2016-03-09 | GSE74819 | GEO

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress [set2]

Project description:We use RNA-Seq to explore the reaction norm of gene expression of larval Anopheles coluzzii and An. merus to a gradient of salinity. After rearing in freshwater, separate pools of 18 h post-hatch larval instars of An. merus and An. coluzzii were briefly (6 h) exposed to one of six water salinities: 0%, 10%, 20%, 30%, 40%, or 50% the salinity of seawater. The last sample (G-50-RC) was split across 5 lanes for sequencing, resulting in total 10 fastq files associated with subparts G-50-RCa, G-50-RCb, G-50-RCc, G-50-RCd, G-50-RCe.

2016-03-09 | E-GEOD-74819 | biostudies-arrayexpress

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress.

Project description:Evolution of osmoregulatory systems is a key factor in the transition of species between fresh- and saltwater habitats. Anopheles coluzzii and Anopheles merus are stenohaline and euryhaline malaria vector mosquitoes belonging to a larger group of sibling species, the Anopheles gambiae complex, which radiated in Africa within the last 2 million years. Comparative ecological genomics of these vector species can provide insight into the mechanisms that permitted the rapid radiation of this species complex into habitats of contrasting salinity. Here, we use RNA-Seq to investigate gene expression differences between An. coluzzii and An. merus after briefly exposing both young and old larval instars of each species to either saltwater (SW) or freshwater (FW). Our study aims to identify candidate genes and pathways responsible for the greater SW tolerance of An. merus. Our results are congruent with the ability of gene induction to mediate salinity tolerance, with both species showing increasing amounts of differential gene expression between SW and FW as salt concentrations increase. Besides ion transporters such as AgAE2 that may serve as effectors for osmoregulation, we also find mitogen-activated protein kinases that may serve in a phosphorylation signalling pathway responding to salinity, and report potential cross-talk between the mosquito immune response and osmoregulation. This study provides a key step towards applying the growing molecular knowledge of these malaria vectors to improve understanding of their ecological tolerances and habitat occupancy.

| S-EPMC4877251 | biostudies-literature

Transcriptomic differences between euryhaline and stenohaline malaria vector sibling species in response to salinity stress

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-03-09 | GSE74820 | GEO

Salinity adaptation in the principal arboviral vector Aedes aegypti characterized by larval cuticle changes

Project description:Aedes aegypti, the principle global vector of arboviral diseases, has been widely regarded to only lay eggs and undergo preimaginal development in fresh water collections. Recent observations however show that it has adapted to develop in anthropogenic brackish water habitats of up to 50% sea water in coastal areas in different continents. This adaptation is characterised by greater salinity tolerance in adult oviposition preference, larvae and changes in sizes of anal papillae. The physiological basis for salinity tolerance in either Ae. aegypti or any of the known salinity-tolerant species of Anopheles malaria vectors is not established. To address this knowledge gap which is of fundamental biological interest and important for control of major diseases we performed RNAseq analysis of gut, anal papillae, and rest of the carcass of Ae. aegypti collected in the field from brackish water (BW) and fresh water habitats (FW) and then maintained as laboratory colonies in BW and FW respectively. We also examined the cuticle structure of larvae, pupae and adult BW and FW Ae. aegypti by microscopy and performed proteomic analysis of the shed cuticles of fourth instar larvae (L4) when they transformed into pupae. The results show that major changes in cuticle structure and composition characterize, and may be the principal factor that permits, the adaptation of Ae. aegypti to brackish water.

2021-04-07 | PXD018397 | Pride

Insights into the epigenomic landscape of the human malaria vector Anopheles gambiae

Project description:We characterize the epigenome of the human malaria vector Anopheles gambiae in midgut cells by mapping the distribution and levels of two post-translational histone modifications, H3K27ac and H3K27me3. These histone profiles were then correlated with levels of gene expression obtained by RNA-seq.

2014-09-04 | GSE59773 | GEO

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