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Mus musculus

ABSTRACT: Gene expression profile in Ronin (Thap11) conditional knock out retina through RNA-sequencing

PROVIDER: PRJNA301605 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2932610.fastq.gz	Fastqsanger.gz
	SRR2932611.fastq.gz	Fastqsanger.gz
	SRR2932612.fastq.gz	Fastqsanger.gz
	SRR2932607.fastq.gz	Fastqsanger.gz
	SRR2932608.fastq.gz	Fastqsanger.gz

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Genome-wide occupancy profiling of Ronin (Thap11) in retinal development

2016-01-19 | GSE74823 | GEO

Gene expression profile in Ronin (Thap11) conditional knock out retina through RNA-sequencing

Project description:Conditional knockout of the transcription factor Ronin (Thap11) in retinal progenitor cells (RPCs) results in a profound failure cell proliferation resulting in a hypoplastic adult retina that also suffers from photoreceptor degeneration. The goal of this study was to determine which genes are deregulated in response to loss of Ronin transcription factor activity in the developing retina. We generated Ronin flox/flox (Control) and Chx10-Cre::GFP+/tg; Ronin flox/flox (CKO) mice, in which Ronin loss occurs specifically within RPCs, and performed RNA-Seq analysis of embryonic day E14.5 (E14.5) retinae. Three independent pools of control and Ronin CKO retinae were collected consisting of a minimum of 10 retinae per pool and total RNA was extracted followed by polyA selection, fractionation (200-500 nucleotide range) and generation of cDNA. The resulting DNA was then used for standard Illumina adaptor ligation and sequencing. This experiment revealed decreased expression of a large group of mitochondrial genes including components of the electron transport chain (ETC), which have been recently implicated as direct regulators of the cell cycle.

2016-01-19 | GSE74822 | GEO

RNA-seq of mouse embryonic stem cells with Ronin/Thap11 knockout

Project description:RNA-seq of Ronin/Thap11 knockout and heterozygous control mouse ES cells to assess the role of Ronin

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Project description:Gene expression profile of retina and pineal gland of NeuroD1 conditional knockout mice

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Project description:mRNA profile of HeLaS3 cell line and AGO2 Knock-Out HeLaS3 subclone

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Project description:The analysis of Ambra1 conditional Knock Out mice

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Mus musculus

Project description:Genome-wide occupancy profiling of Ronin (Thap11) in retinal development

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Project description:Gene expression profiles of Rif1 conditional knock-out large T immortalized mouse embryonic fibroblast

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