Homo sapiens
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ABSTRACT:
PROVIDER: PRJNA304033 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Analysis of gene expression patterns in cytomegalovirus infected human fibroblasts for two cultures with differing sensitivity to the virus Total RNA obtained from 2 human fibroblast lines with differing sensitivity to CMV infection. For each line 3 types of samples are compared: pre-experimantal norm, 3 hours after infection, 3 hours without infection.
2015-11-25 | E-GEOD-75366 | biostudies-arrayexpress
Project description:CMV infected human fibroblasts in two cultures with differing sensitivity to the virus
| PRJNA304032 | ENA
Project description:Analysis of microRNA expression patterns in cytomegalovirus infected human fibroblasts for two cultures with differing sensitivity to the virus Non-coding RNAs were cloned from total RNA extracts obtained from 2 human fibroblast lines with differing sensitivity to CMV infection. For each line 3 types of samples are compared: pre-experimantal norm, 3 hours after infection, 3 hours without infection.
2015-11-25 | E-GEOD-75305 | biostudies-arrayexpress
Project description:Analysis of gene expression patterns in cytomegalovirus infected human fibroblasts for two cultures with differing sensitivity to the virus
2015-11-25 | GSE75366 | GEO
Project description:Analysis of microRNA expression patterns in cytomegalovirus infected human fibroblasts for two cultures with differing sensitivity to the virus
2015-11-25 | GSE75305 | GEO
Project description:microRNA expression analysis of CMV infected human fibroblasts in two cultures
| PRJNA304028 | ENA
Project description:Plasmacytoid dendritic cells (pDCs) can rapidly produce interferons and other soluble factors in response to extracellular viruses or virus mimics such as CpG-containing DNA. pDCs can also recognize live cells infected with certain RNA viruses, but the relevance and functional consequences of such recognition remain unclear. We studied the response of primary DCs to the prototypical persistent DNA virus, the human cytomegalovirus (CMV). Human pDCs responded poorly to free CMV but strongly to live CMV-infected fibroblasts, in a process that involved integrin-mediated adhesion, transfer of viral DNA to pDCs and its recognition through TLR9. Compared to transient polyfunctional responses to CpG or free influenza virus, pDC response to CMV-infected cells was long-lasting, dominated by the production of type I (IFN-I) and type III (IFN-III) interferons, and lacked diversification into functionally distinct populations. Similarly, pDC activation by influenza-infected lung epithelial cells was highly efficient, prolonged and dominated by interferon production. Prolonged pDC activation by CMV-infected cells facilitated the activation of natural killer cells that are critical for CMV control. Finally, patients with CMV viremia harbored phenotypically activated pDCs and increased levels of IFN-I and IFN-III in circulation. Thus, recognition of live infected cells is a common mechanism of virus detection by pDCs that elicits a unique antiviral response program.
2021-03-01 | GSE163707 | GEO
Project description:Cytomegalovirus (CMV) strains with different in vivo and in vitro characteristics may induce different patterns of cellular gene expression. CMV strain fhCMV-H, which spreads rapidly through cell culture, was isolated from a hematopoetic stem cell transplant patient who died of CMV-mediated marrow failure. In contrast fhCMV-T was isolated from a patient who survived CMV complications and does not spread rapidly in vitro. Fibroblasts were infected with both strains, which had been engineered to express GFP upon cellular infection, and the cellular gene expression profile was compared to that of noninfected controls at 24h. The gene expression profile of HFF exposed to UV-inactivated virus from the 2 strains was also tested, to differentiate the effects of the initial engagement with virus coat proteins such as glycoprotein B and gene modulation by active virus. Keywords = cytomegalovirus, glycoprotein B Keywords: repeat sample
2003-09-09 | GSE453 | GEO
Project description:We constructed two independent small RNA libraries from leaves of mock and Cucumber mosaic virus (CMV) infected tomatoes, respectively, and sequenced with a high-throughput Illumina Solexa system. Based on sequence analysis and hairpin structure prediction, a total of 50 known miRNAs (32 families) and 568 potentially candidate miRNAs (PC-miRNAs) were firstly identified in tomato, with 12 known miRNAs and 154 PC-miRNAs supported by both the 3p and 5p strands. Comparative analysis revealed 79 miRNAs (including 15 novel tomato miRNAs) and 40 PC-miRNAs were differentially expressed between the two libraries. Among these virus responsive miRNAs, expression patters of some novel tomato miRNAs and PC-miRNAs in mock and in CMV-Fny infected tomatoes were further validated by qRT-PCR. Moreover, we revealed 563 potential targets for 66 tomato miRNAs by the recently developed degradome sequencing approach, including 124 targets for 7 new tomato miRNAs and 97 targets for 24 PC-miRNAs. Target annotation for the newly identified miRNA and PC-miRNAs indicated that they were involved in multiple biological processes, including transcriptional regulation and virus resistance. Gene ontology analysis of these target transcripts demonstrated that stress response- and photosynthesis-related genes were most affected in CMV-Fny infected tomatoes.
2014-10-20 | GSE56974 | GEO
Project description:We constructed two independent small RNA libraries from leaves of mock and Cucumber mosaic virus (CMV) infected tomatoes, respectively, and sequenced with a high-throughput Illumina Solexa system. Based on sequence analysis and hairpin structure prediction, a total of 50 known miRNAs (32 families) and 568 potentially candidate miRNAs (PC-miRNAs) were firstly identified in tomato, with 12 known miRNAs and 154 PC-miRNAs supported by both the 3p and 5p strands. Comparative analysis revealed 79 miRNAs (including 15 novel tomato miRNAs) and 40 PC-miRNAs were differentially expressed between the two libraries. Among these virus responsive miRNAs, expression patters of some novel tomato miRNAs and PC-miRNAs in mock and in CMV-Fny infected tomatoes were further validated by qRT-PCR. Moreover, we revealed 563 potential targets for 66 tomato miRNAs by the recently developed degradome sequencing approach, including 124 targets for 7 new tomato miRNAs and 97 targets for 24 PC-miRNAs. Target annotation for the newly identified miRNA and PC-miRNAs indicated that they were involved in multiple biological processes, including transcriptional regulation and virus resistance. Gene ontology analysis of these target transcripts demonstrated that stress response- and photosynthesis-related genes were most affected in CMV-Fny infected tomatoes. Examination of small RNAs and their targets in mock and CMV-Fny infected tomatoes.
2014-10-20 | E-GEOD-56974 | biostudies-arrayexpress