Project description:This study investigated the epigenetic mark of DNA methylation in the medial prefrontal cortex (mPFC) as a function of age and cognition. Young and aged F344 rats were characterized in a cognitive flexibility task, set shifting, and whole-genome bisulfite sequencing was performed in an Illumina system. The results indicate that differential methylation was linked to the expression of genes within functional categories which may mediate impaired cognition in aging. Differences in aging included hypermethylation of genes linked to synaptic function and GTPase activity. Further, age-related cognitive flexibility impairment was correlated to hypermethylation of synaptic, postsynaptic density and ion channel activity genes.
Project description:Transcriptomic analysis of prefrontal medial cortex, hippocampus and nucleus accumbens of alcohol-preferring and nonpreferring rats
Project description:This study examined the proteome profile in the hippocampus, medial prefrontal cortex, and striatum of APPswe/PS1dE9 transgenic mice (APP/PS1) model of Alzheimer’s disease compared to wild-type mice. The effect of tocotrienol-rich fraction (TRF), a mixture of vitamin E analogs derived from palm oil supplementation on the proteome profile of APP/PS1 mice hippocampus, medial prefrontal cortex, and striatum was also investigated. The analysis was performed using ultrahigh-performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry. This study was in hoped to understand the mechanisms of Alzheimer’s disease at proteome level, and pre-emptive activity of TRF to combat the disease.
Project description:Gene expression changes in the medial prefrontal cortex and nucleus accumbens following abstinence from cocaine self-administration
Project description:This study investigated proteome profile in the hippocampus, medial prefrontal cortex (mPFC), and striatum of 14, 18, 23, and 27 months old rats in order to see the effect of aging on proteins in these regions. Using ultrahigh performance liquid chromatography coupled with Q Exactive HF Orbitrap mass spectrometry, we identified 1074 proteins in the hippocampus, 871 proteins in the mPFC, and 241 proteins the striatum. Ninety-seven, 25, and 5 of these proteins were differentially expressed with age in the hippocampus, mPFC, and striatum, respectively. Overall, aging causes divergent protein changes in specific brain regions, with the most prominent changes observed in the late-aged.
