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Homo sapiens

ABSTRACT: Genomic binding profiles of ERa in MCF-7 and T47D control or enhancer588-targeted cells

PROVIDER: PRJNA305318 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2980442.fastq.gz	Fastqsanger.gz
	SRR2980443.fastq.gz	Fastqsanger.gz
	SRR2980444.fastq.gz	Fastqsanger.gz
	SRR2980445.fastq.gz	Fastqsanger.gz
	SRR2980446.fastq.gz	Fastqsanger.gz

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Genomic binding profiles of ERa in MCF-7 and T47D control or enhancer588-targeted cells

Project description:Estrogen Receptor a (ERa) bindning to DNA was profiled by ChIP-seq in MCF-7 and T47D cells transduced with either control sgRNA, or sgRNA targeting a specific enhancer region (enhancer588). ERa in MCF-7 and T47D control or enhancer588-targeted cells

2015-12-14 | E-GEOD-75779 | biostudies-arrayexpress

Genomic binding profiles of ERa in MCF-7 and T47D control or enhancer588-targeted cells

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Project description:Here, we report that in T47D breast cancer cells 50pM progestin is sufficient to activate cell cycle entry and the progesterone gene expression program in breast cancer cells. At this concentration, equivalent to the progesterone blood levels found around the menopause, PR binds only to 2,800 genomic sites, which are accessible to ATAC cleavage prior to hormone. These Highly-Accessible PR Binding sites (HAPRBs) are surrounded by well-organized nucleosomes and exhibit breast enhancer features, including higher estrogen receptor (ERa), FOXA1 and BRD4 occupancy. Although, HAPRBs are enriched in RAD21 and CTCF, PR binding is the driving force for the most robust interactions with hormone-regulated genes. HAPRBs show higher frequency of 3D contacts among themselves than with other PRBs, indicating co-localization in similar compartment. Gene regulation via HAPRBs is independent of classical co-regulators and ATP-activated remodelers, relaying mainly on MAP kinase activation that enables PR nuclear engagement. HAPRBs are also preferentially occupied by PR and ERa in breast cancer xenografts derived from MCF-7 cells as well as from patients (PDXs), indicating their usefulness as potential targets for therapeutic intervention.

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Transcriptome changes in MCF-7 and T47D breast cancer cells after four day incubation in glucose deprived medium and beta-hydroxybutyrate

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Homo sapiens

Project description:Estrogen effects on MCF-7 breast cancer cells co-expressing ERa and ERb

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