Project description:Experiment to estimate mutatational variance of gene expression in Drosophila melanogaster at two times in development using 12 mutation accumulation lines. Keywords = evolution Keywords = quantitative genetics Keywords = Drosophila Keywords = mutation Keywords: other
Project description:Experiment to estimate mutatational variance of gene expression in Drosophila melanogaster at two times in development using 12 mutation accumulation lines. Keywords = evolution Keywords = quantitative genetics Keywords = Drosophila Keywords = mutation Each of 12 lines measured 8 times in each of two stages. For each stage, the design has two hexagons with 6 interior connections (=hybridizations) (all except opposite nodes). Each line in one hexagon is connected to one line in the other hexagon and that line's opposite. Each line is also connected to itself twice in the other stage. Dyes are balanced.
Project description:25 lines are maintained with 10 females and 10 males to accumulate mutations from a single inbred strain DGRP_360. Mutations are allowed to accumulate for 60 generations. At generation 60, 10 3-5 day old adult flies, sex separately, are pooled and whole body RNA are extracted and profiled for gene expression. This data set allows estimation of mutational variance in gene expression.
