Project description:Drosophila translationally controled tumor protein (dTCTP) is important to repair double stranded DNA breaks in cell nucleus. However, besides damaged DNA loci, dTCTP is also located in interbands region of polytene chromsomes of salivary gland tissues. To address whether dTCTP is involved in transcriptional regulation process, we performed microarrays in salivary gland tissues from dTCTP mutants and w[1118] wildtype control. Salivary glands tissues were dissected and collected from 3rd instar larvae or each genotype for RNA extraction and hybridization on Affymetrix microarrays.
Project description:In order to evaluate the global transcriptomic changes in endoreplicating cells in Drosophila melanogaster we performed RNA-Seq. Endoreplicating cells (salivary glands) and mitotic cycling cells (brains and imaginal discs) were harvested from feeding 3rd instar larvae and mRNA levels were compared. Consistent with our previous results, we found that endoreplicating cells repress genes regulated by the E2F1 transcription factor as well as genes regulated by the Myb-MuvB (MMB) transcription factor complex.
Project description:Affymetrix microarray to detect changes in gene expression between lgl27S3/lglE2S31 and FRT82B larvae 4-day old 3rd instar lgl27S3/lglE2S31 larvae were compared to 0-day old 3rd instar FRT82B larvae
Project description:Single channel custom oligonucleotide array of 147 microRNAs to detect changes in expression of a lgl-hypomorph mutant versus wild-type 0, 3, and 5 day old 3rd instar lgl-hypomorph larvae were compared to 0 day 3rd instar wild-type larvae to test for differences in microRNA expression
Project description:Drosophila translationally controled tumor protein (dTCTP) is important to repair double stranded DNA breaks in cell nucleus. However, besides damaged DNA loci, dTCTP is also located in interbands region of polytene chromsomes of salivary gland tissues. To address whether dTCTP is involved in transcriptional regulation process, we performed microarrays in salivary gland tissues from dTCTP mutants and w[1118] wildtype control.
Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands.
Project description:We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein.
Project description:ChIP was performed to identify regions of gDNA bound by orc2 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that ORC does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands.
Project description:ChIP was performed to identify regions of gDNA bound by RNA polymerase II in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that RNAPolII does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands.
Project description:The major H4K16 acetylase MOF has been found in two complexes, the Male-Specific Lethal (MSL) and the Non-Specific Lethal (NSL) complex. The latter one consists of at least 7 members: NSL1, NSL2, NSL3, MBD-R2, MCRS2, WDS, and MOF. To investigate it's function, we performed genome-wide profiling of NSL3 and MBD-R2 in S2 cells. In addition, we obtained ChIP-Seq profiles for NSL1 and MCRS2 from 3rd instar larvae salivary glands that can be accessed via the accession number E-MTAB-214. This submission is also related to E-MTAB-1084.
