Project description:Our previous data obtained by using immunohistochemistry showed, that Fgf10+/- (50% Fgf10 expression compared to WT) in hyperoxic condition at postnatal day 3 (P3) compared to WT has less vessel count in the lung and less muscularization of small capillaries in the lung. Furthermore, Fgf10+/- showed a drastic increase in mortality upon hyperoxic lung injury. Main question to be answer by this experiment is as followed: Does AEC II from Fgf10+/- mice in normoxia show different expression profiles at P3 compared to AEC II from WT? To adress this question we harvest lungs at P3 from WT and Fgf10+/- . For this the mice were sacrificed by Ketamin/ Dormitor ip, lungs were perfused transcardiac with PBS and directly frozen in liquid nitrogen.

2016-12-31 | GSE76300 | GEO

Gene expression profiles in AEC II during hyperoxia exposure and recovery

Project description:In this study, type II alveolar epithelial cells (AEC II) were isolated from normal, hyperoxia exposed (day 0) and recovery (day 1,3,5 and 7) rats with high purity (~95%). Total RNA from isolated cells were used for dual color DNA microarray hybridization with 3DNA 50 kit version 2. Keywords: time-course

2010-06-11 | E-GEOD-2707 | biostudies-arrayexpress

Fgf10 knock-down effects in hyperoxia

Project description:Our previous data obtained by using immunohistochemistry showed, that Fgf10+/- (50% Fgf10 expression compared to WT) in hyperoxic condition at postnatal day 3 (P3) compared to WT has less vessel count in the lung and less muscularization of small capillaries in the lung. Furthermore, Fgf10+/- showed a drastic increase in mortality upon hyperoxic lung injury. Main question to be answer by this experiment is as followed: Does Fgf10+/- mice after hyperoxia from P0-P3 show different expression profiles at P3 compared to WT? To adress this question we harvest lungs at P3 from WT and Fgf10+/- after hyperoxia treatment from P0-P3. For this the mice were sacrificed by Ketamin/ Dormitor ip, lungs were perfused transcardiac with PBS and directly frozen in liquid nitrogen.

2019-05-01 | GSE113102 | GEO

Hyperoxia in FGF10 knock-down mice [Lung]

Project description:Our previous data obtained by using immunohistochemistry showed, that Fgf10+/- (50% Fgf10 expression compared to WT) in hyperoxic condition at postnatal day 3 (P3) compared to WT has less vessel count in the lung and less muscularization of small capillaries in the lung. Furthermore, Fgf10+/- showed a drastic increase in mortality upon hyperoxic lung injury. Main question to be answer by this experiment is as followed: Does Fgf10+/- mice after hyperoxia from P0-P3 show different expression profiles at P3 compared to WT? To adress this question we harvest lungs at P3 from WT and Fgf10+/- after hyperoxia treatment from P0-P3. For this the mice were sacrificed by Ketamin/ Dormitor ip, lungs were perfused transcardiac with PBS and directly frozen in liquid nitrogen.

2016-12-31 | GSE76301 | GEO

Gene expression profiles in AEC II during hyperoxia exposure and recovery

2006-06-06 | GSE2707 | GEO

Fgf10 knock-down effects in normoxia

Project description:Fgf10 knock-down effects in normoxia

| PRJNA450017 | ENA