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Homo sapiens

ABSTRACT: ATAC-seq data from EB-HSPC after AM580 treatment compared to DMSO-treated EB

PROVIDER: PRJNA308423 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3095971_1.fastq.gz	Fastqsanger.gz
	SRR3095971_2.fastq.gz	Fastqsanger.gz
	SRR3095972_1.fastq.gz	Fastqsanger.gz
	SRR3095972_2.fastq.gz	Fastqsanger.gz
	SRR3095973_1.fastq.gz	Fastqsanger.gz

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Homo sapiens

Project description:RNA-seq expression data from EB-HSPC after AM580 treatment compated to DMSO-trated and FL-HSPCs

| PRJNA308426 | ENA

RNA-seq expression data from EB-HSPC after AM580 treatment compated to DMSO-trated and FL-HSPCs

Project description:RA signalling regulated endothelial to hematopoietic transition and HSC generation. EB- or FL-derived HSPC were profiled before (d0) or after (d6) 6 days of treatment with 0.2uM AM580 on OP9, and after 6 additional days of expandion of OP9 (d12) without treatment.

2016-05-16 | E-GEOD-76682 | biostudies-arrayexpress

Effect of T0901317, AM580 or T0901317+AM580 on monocyte gene expression

Project description:The aim of this study was to investigate the effect of T0901317 LXR agonist on Retinoic Acid Receptor (RAR) family gene expression. T0901317 alone increase RARalpha gene expression in monocytes and the effects of the co treatment of the cells with T0901317 and a specific RARalpha agonist (AM580) were further studied. For this monocytes were treated for 24h with DMSO or T0901317 to induce RARalpha expression and in a second step cells were treated or not with AM580 for another 24h period. Gene expression was analysed for all the treatments (T0901317, AM580 or T0901317+AM580) and we focussed on genes which expression was synergitically induced by the co treatment as compared to the cells treated either by T0901317 or AM580.

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Effect of T0901317, AM580 or T0901317+AM580 on monocyte gene expression

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ATAC-seq_experiment in RKO cell line under low replication stress

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ATAC-Seq on Cohesin mutant HSPCs

Project description:Understanding the impact of cohesin mutations on HSPC chromatin structure We examined chromatin structure using ATAC-seq in CD34+ enriched-human HSPC that were transduced with either cohesin WT, mutant or empty vector controls

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Transcriptomic analysis of MERS-CoV infected Calu-3 cell with or without AM580 treatment

Project description:We report a broad spectrum antiviral drug AM580, RNA-Seq technology was used to compare the host gene mRNA expression between different groups to indicate the host pathway changes upon MERS-CoV infection and AM580 treatment.

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RNA-seq expression data from EB-HSPCs after HOXA7 overexpression

Project description:HOXA7 regulates FL-HSPC self-renewal in vitro and in vivo. We profiled EB-HSPCs after HOXA7 overexpression (EB-HOXA7), or with a control vector (EB-CTR), to assess the gene expression programs regulated by HOXA7. CD34+CD38-CD43+CD90+ HSPCs were infected with lentiviral FUGW vector either empty (FUGW-GFP) or encoding HOXA7(FUGW-GFP-HOXA7) protein. Cells were expanded on op9 for 15 days and than sorted for GFP HSPC immunophenotype.

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Runx+ HSPC, kdrl+ endothelial, and negative cells sorted from DMSO- or Lycorine-treated 3 dpf zebrafish embryos

Project description:The zebrafish is a powerful model for the study of hematopoietic stem and progenitor cells (HSPC). We have developed a novel HSPC-specific transgenic line (Runx1+23:GFP). We have used this line in time-lapse live imaging studies to track the migration of HSPC during development. We have also performed a chemical genetic screen to find small molecules that modulate HSPC numbers during development. Treating embryos from 2-3 days post fertilization (2-3 dpf) then fixing for in situ staining with HSPC probes cmyb and runx1, we found the compound lycorine increased HSPC numbers. Applying this compound during time-lapse live imaging showed increased accumulation of Runx+ HSPC in the caudal hematopoietic tissue (CHT). Treatment from 2-3 dpf, then washing off the compound, had a sustained effect on the size of the HSPC with Runx+ numbers higher at 5 and 7 dpf. We have performed microarray analysis to elucidate the molecular changes within HSPC and endothelial cells after Lycorine treatment. We treated Runx1+23:GFP;kdrl:DsRed2 embryos from 2-3 dpf with 75 uM lycorine in 1% DMSO. We then dissociated the embryos and sorted the Runx+ GFP cells, the kdrl+ DsRed2 cells, and the non-fluorescent negative cells from the total embryo as a comparator population. Total RNA was amplified and biotin labled for hybridization on Affymetrix microarrays. 18 samples were collected and analyzed. There are 3 biological replicates. There are 3 cell type populations: 1) Runx+ HSPC; 2) kdrl+ endothelial cells; 3) non-fluorescent negative cells. There are cell populations from dissociated Lycorine-treated embryo pools, and control DMSO-treated embryo pools.

2015-01-15 | E-GEOD-56015 | biostudies-arrayexpress

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Project description:ATAC-seq from MCF-7 cells treated with Nutlin-3a or DMSO control

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