Project description:Transcriptional profiling by next generation sequencing of the spleen and splenic macrophages in the hamster model of visceral leishmaniasis
Project description:Non–alcoholic fatty liver disease (NAFLD) is high prevalent in worldwide and associated with chronic kidney disease (CKD). Infection with Opisthorchis viverrini (Ov) infection and consumption of high fat and high fructose (HFF) exacerbates NAFLD to nonalcoholic steatohepatitis in hamsters. Here, we aimed to investigate the effect a combination of HFF diet and Ov infection on kidney pathology via alteration of gut microbiome and proteome in hamster.
Project description:Hyperlipidemia is one of high risk factors for cardiovascular disease (CVD), whose regulation is known to be mediated also by microRNA-mediated mechanisms. Therefore, the analysis of transcriptomics data would gain insights into the molecular mechanisms implicating microRNAs (miRNAs) behind the atherogenic hyperlipidemia. The goal of our study was to profile the specific miRNAs expression associated with atherogenic hyperlipidemia. To this aim, we performed miRNAs microarray analysis for different tissues (liver, left ventricle of the heart, small intestine and blood mononuclear cells) obtained from the experimental model of high-fat diet induced hyperlipidemic hamsters (Mesocricetus auratus). The microarray data were obtained using miRCURY LNA microRNA arrays 7 th generation platform with miRBase 19 (Exiqon, Denmark), using total RNA isolated from hyperlipidemic (HL) and normolipidemic (NL) hamsters? tissues and blood cells. The animals were fed for 12 weeks either standard rodent chow supplemented with 3% cholesterol and 15% unsalted butter (HL) or standard rodent chow (NL), with free access to food and water.
