Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA308590 | ENA |
REPOSITORIES: ENA
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Project description:X. albilineans is one of the most important phytobacteria species which affect sugarcane production. Volatile organic compounds (VOCs) produced by microorganisms may have a noteworthy role in the control of plant diseases. Thus, this study investigated VOC-producing soil bacteria with an antagonistic effect against X. albilineans and evaluated the molecular mechanisms of growth inhibition trigged by the volatile dimethyl disulfide (DMDS). The comparative transcriptomic data of X. albilineans treated with DMDS showed that several metabolic pathways are up-regulated, such as two-component system, flagellar assembly, chemotaxis and bacterial secretion system. Interesting, although the ethanol used as DMDS solvent did not inhibit X. albilineans growth, it triggers a similar gene up-regulation and somehow, the phytopathogen can deal with this harmful compound better than DMDS.
2025-01-31 | GSE246249 | GEO
Project description:Plants are targets of volatile organic compounds (VOCs) released as a part of plant-plant communication, within-plant self-signaling and plant-microbe interactions. Therefore, understanding VOC perception and downstream signaling is vital for unraveling the mechanisms behind information exchange in plants, which remain largely unexplored. Using the hormone-like function of volatile terpenoids in reproductive organ development as a system with a visual marker for communication, we demonstrated that among the four petunia karrikin-insensitive receptors, PhKAI2ia stereo-specifically perceives the (-)-germacrene D signal, triggering a karrikin-like signaling cascade. This study provides new insights into plant olfaction, uncovers the role(s) of the unique intermediate clade of KAI2 receptors, illuminates the involvement of KAI2ia-dependent signaling pathway in volatile communication and gives insight into the long-standing question about the nature of potential endogenous karrikin-like ligand(s).
2024-02-07 | GSE245080 | GEO
Project description:Inhalation is the most relevant route of volatile organic chemical (VOC) exposure; however, due to unique challenges posed by their chemical properties and poor solubility in aqueous solutions, in vitro chemical safety testing is predominantly performed using direct application dosing/submerged exposures. To address the difficulties in screening toxic effects of VOCs, our cell culture exposure system permits cells to be exposed to multiple concentrations at air-liquid interface (ALI) in a 24-well format. ALI exposure methods permit direct chemical-to-cell interaction with the test article at physiological conditions. In the present study, BEAS-2B and primary normal human bronchial epithelial cells (pHBEC) are used to assess gene expression, cytotoxicity, and cell viability responses to a variety of volatile chemicals including acrolein, formaldehyde, 1,3-butadiene, acetaldehyde, 1-bromopropane, carbon tetrachloride, dichloromethane, and trichloroethylene. BEAS-2B cells were exposed to all the test agents, while pHBECs were only exposed to the latter four listed above. The VOC concentrations tested elicited only slight cell viability changes in both cell types. Gene expression changes were analyzed using benchmark dose (BMD) modeling. The BMD for the most sensitive gene set was within one order of magnitude of the threshold-limit value reported by the American Conference of Governmental Industrial Hygienists, and the most sensitive gene sets impacted by exposure correlate to known adverse health effects recorded in epidemiologic and in vivo exposure studies. Overall, our study outlines a novel in vitro approach for evaluating molecular-based points-of-departure in human airway epithelial cell exposure to volatile chemicals.
2022-04-17 | GSE199794 | GEO
Project description:Detection of Volatile Organic Compounds (VOC) directly from tissue by headspace analysis (skin, surgery material, other tissue) and exhaled breath is feasible using affordable user-friendly novel nano-chemo sensors that can accurately be used for screening and monitoring purpose
| 2250131 | ecrin-mdr-crc
Project description:To obtain deeper understanding of atmospheric dynamics of the potent greenhouse gas methane, controlling factors of methanotrophs, as the sole biological methane sink, is necessary. Recent research has revealed complex interactions between methanotrophs and heterotrophs, involving volatile organic compounds (VOCs). In environments with high methane concentrations VOC-mediated interactions significantly influence methane cycling and emissions. Here, we employed a multidisciplinary approach, utilizing proteomics, volatile analysis, and measurements of bacterial growth and methane oxidation to elucidate underlying mechanisms of VOC-mediated interactions between heterotrophs and methanotrophs. The results demonstrate that specific VOCs, like dimethylpolysulfides, released by heterotrophic bacteria can inhibit growth and methane uptake of methanotrophs, while other VOCs had the opposite effect. Proteomics analysis revealed differential protein expression patterns depending on exposure to the volatolome of a heterotrophic bacterium or with CO2 added, which was most pronounced with the particulate and soluble methane monooxygenase. The current study demonstrated potential biotic modulation of methanotrophy without direct contact, caused by VOC or CO2 from respiration, or both, with a proteomic response. Although further research is needed to elucidate the specific mechanisms involved, it is clear that methanotroph-heterotroph interactions need to be investigated closer to informs strategies for mitigating emission of the greenhouse gas methane.
2024-08-08 | PXD051964 | Pride
Project description:Plant volatile organic compounds (VOCs) play a key role in defense against herbivores and in mediating multitrophic interactions. Yet, how surrounding plant diversity modulates these responses in forest trees remains poorly understood. We examined the effects of tree species richness on induced VOC emissions and arthropod predation in Quercus petraea in a biodiversity-ecosystem functioning experiment. Individual branches were repeatedly induced with methyl jasmonate and paired with non-induced controls. VOCs were collected using PDMS tubes after the first and last induction rounds and analyzed by TD-GC-MS. Predation was quantified using artificial clay caterpillars. Jasmonate induction strongly altered VOC profiles, with effects increasing after repeated treatments. Tree species richness modulated VOC composition during the first sampling period, particularly affecting sesquiterpenes. However, absolute VOC compound class emissions did not explain variation in arthropod predation probability, while class-level composition showed only weak and inconsistent effects across sampling periods. These results suggest that indirect defense outcomes in terms of predator responses are unlikely to depend on overall VOC emission strength or broad chemical classes but may instead be mediated by fine-scale properties of volatile blends.
2026-02-17 | MSV000100870 | MassIVE
Project description:When aboveground parts of intact plants are exposed to volatile organic compounds emitted from neighboring con-/heterospecific plants that are artificially damaged or damaged by herbivores, the resistant responses are induced in the plants. Differential responses of plants to enantiomers of the same volatile compound have also been reported in Arabidopsis: the root became shorter when Arabidopsis seedlings are exposed to aerial borneol, and the dose-dependent root length reduction was significantly different between (+)- and (-)-borneol. We identified (+)-borneol dependent inductive genes in Arabidpsis in this transcriptome analysis.
2023-04-01 | GSE180220 | GEO
Project description:Coniferous trees use the oleoresin, a mixture of non-volatile non-volatile diterpene acids and a large (20-50%) volatile fraction of mono- and sesquiterpenes, as a defence tool against damaging herbivores and pathogens. Volatilization of monoterpenes increases viscosity of oleoresin and finally leading to polymerization of resin and a formation of protective solid plug. Resin storing conifers form an important source of volatile organic compounds (VOCs) mainly dominated by volatile monoterpenes. In the atmosphere, conifer VOCs react with ozone and OH and nitrous oxide radicals forming secondary organic compounds. This project seeks a better understanding of the genetic control of conifer defences and the role of monoterpenoid compounds under biotic stresses.
2015-04-01 | GSE60383 | GEO