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Homo sapiens

ABSTRACT: Human cervical cells HELP-tagging cytosine methylation data

PROVIDER: PRJNA309179 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3106690.fastq.gz	Fastqsanger.gz
	SRR3106700.fastq.gz	Fastqsanger.gz
	SRR3106710.fastq.gz	Fastqsanger.gz
	SRR3106720.fastq.gz	Fastqsanger.gz
	SRR3106730.fastq.gz	Fastqsanger.gz

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Homo sapiens immortalized MSCs: KP vs. 3A6

Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.

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Project description:We pursued the hypothesis that epigenetic regulators of transcription are involved in both the development and the progression of HPV-associated CIN. Using HELP-tagging, we performed the most comprehensive study to date of DNA methylation in HPV-associated cervical neoplasia, testing ~2 million loci throughout the human genome in biopsies from 78 HPV+ women, identifying changes starting in early CIN and maintained through carcinogenesis. We identified loci at which DNA methylation is consistently altered, beginning early in the course of neoplastic disease and progressing with disease advancement. DNA methylation profiles for cervical biopsies of 19 normals, 20 CIN1, 16 CIN2/3, and 23 cervical cancers.

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