Project description:The goal of this study was to perform RNA-seq expression analysis on Solanum lycopersicum cv. M82 X S. pennellii introgression lines, deriving expression Quantitative Trait Loci which were analyzed together with pre-existing genomic and phenotypic data to define genes and regulatory pathways controlling tomato root development and observed natural variation. We completed the RNAseq expression profiling analysis and developed a tool to display this information graphically in collaboration with Nicholas Provart at the University of Toronto: http://bar.utoronto.ca/efp_tomato/cgi-bin/efpWeb.cgi?dataSource=ILs_Root_Tip_Brady_Lab To identify candidate genes and pathways we focussed on one root growth trait, root growth angle, and identified two statistically significant genomic regions within tomato root growth angle QTL containing two candidate genes that likely control the gravitropic setpoint angle (CDC73 and PAP27), both of which are conserved between Arabidopsis and tomato, and which we tested using transgenic lines of the Arabidopsis orthologs. A possible regulatory role for suberin in root growth angle control was also identified.

2018-01-01 | GSE87162 | GEO

Small RNA (sRNA) sequencing data from pre-meiotic and meiotic anthers of Asparagus

Project description:Purpose: To study pre-meiotic (21-nt) and meiotic (24-nt) phasiRNA pathways in non-grass monocots Methods: Anthers were dissected using a 2 mm stage micrometer in a stereo microscope, and immediately frozen in liquid nitrogen until total RNA isolation was performed. Small RNA, mRNA libraries were generated using short-read (Illumina) and Single Molecule Real Time SMRT (PacBio) sequencing approaches. Stages were assigned based on the morphology of archesporial (AR) and tapetal cells of A. officinalis (Asparagus) anthers.

2018-07-07 | GSE112800 | GEO

RNA-Seq of wild type and osmtd2 mutant rice anthers

Project description:Rice anthers at anthesis stage from the wild type and osmtd2 mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.

2021-06-30 | E-MTAB-10412 | biostudies-arrayexpress

RNA-Seq of wild type and MADS triple mutant anthers

Project description:Rice anthers at anthesis stage from the wild type and MADS triple mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.

2022-01-01 | E-MTAB-11246 | biostudies-arrayexpress

Small RNA (sRNA) sequencing data from pre-meiotic and meiotic anthers of H. lilioasphodelus (Daylily)

2018-07-07 | GSE112799 | GEO

RNA-Seq of wild type and tape-1 mutant rice anthers

Project description:Rice anthers at anthesis stage from the wild type and tape-1 mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.

2022-12-31 | E-MTAB-10439 | biostudies-arrayexpress

RNA sequencing of control and OsRac6 knockout mutant in mature rice anthers

Project description:Rice anthers at anthesis stage from the wild type and osrac6-1 mutant anther (Dongjin cultivar) We collected the sample from our field and immediately froze the samples with liquid nitrogen.

2024-07-02 | E-MTAB-13965 | biostudies-arrayexpress

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