ABSTRACT: Gene expression analysis in the peripheral blood of baboons for early prediction of the later occurring hematological ARS using human Agilent SurePrint G3 Microarrays, v2 (8x60K)
Project description:We performed expression profiling using microarray technology. The expression profiling identified genes or mechanism potentially regulating the proliferation of human lung cancer cells by BoxA of HMGB1 and Alu-siRNA transfection. The RNAs of our experiment were hybridized with Agilent SurePrint G3 Human GE 8X60K, V3 Microarrays (Agilent®).
Project description:PBMCs from major depressive episode (MDE) subjects were obtained at two time points: during a severe episode of depression and eight week later after clinical remission. PBMCs from sex- and age-machted controls were also obtained at an eight weeks interval. PBMC total RNAs from MDE and control subjects were profiled after hybridization with Agilent SurePrint G3 Human GE 8x60K Microarray to identify blood biomarkers of MDE.
Project description:MicroRNAs are useful biomarkers for various disease states, and their preservation in formalin-fixed, paraffin-embedded (FFPE) tissue makes them particularly useful for clinicogenetic studies. Although global microRNA expression in FFPE samples is routinely measured with microarrays, the utility of RNA sequencing for such profiling has yet to be established. In this study, to appraise the suitability of RNA sequencing, microRNAs in RNA from pathologic stage I lung adenocarcinoma FFPE samples were quantified with 8x60K Agilent® SurePrint™ G3 Human miRNA 8x60k (release 16.0) microarray and Illumina® HiSeq™ 2000 sequencing platforms.
Project description:To identify genes hypermethylated and transcriptionally downregulated, we have employed the Illumina Infinium HumanMethylation450 BeadChip and Agilent SurePrint G3 Human Gene Expression 8x60K. And we validated selected genes in cases and controls. eventually we estalished CIMP of MDS.
Project description:Diet-induced obesity (DIO) is associated with a decreased oral fat detection in rodents. This blunted gustatory detection has been explained by an impairment of the lipid-mediated signaling in taste bud cells (TBC). However, factors responsible for this defect remain elusive. To better understand the mechanisms involved, circumvallate papillae from Lean, DIO and former DIO mice were analyzed with a transcriptomic approach. [using Agilent Sureprint G3 Mouse microarrays (8x60K, design 028005)]
Project description:SurePrint G3 Human Gene Expression 8x60k oligonucleotide microarrays (Design ID: 028004,Agilent) were used to characterize gene expression profiles of A549 cells exposed to different concentrations of HBCD (hexabromocyclododecane) for 24 hours.
