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Mus musculus

ABSTRACT: RNA-seq data from murine eGFP+ relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre splenic germinal center B cells purified by fluorescent activated cell sorting.

PROVIDER: PRJNA310183 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3133760_1.fastq.gz	Fastqsanger.gz
	SRR3133760_2.fastq.gz	Fastqsanger.gz
	SRR3133761_1.fastq.gz	Fastqsanger.gz
	SRR3133761_2.fastq.gz	Fastqsanger.gz
	SRR3133762_1.fastq.gz	Fastqsanger.gz

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Expression data from murine eGFP+ relfl/flCg1-Cre and eGFP-Cg1-Cre splenic germinal center B cells purified by fluorescent activated cell sorting.

Project description:Gene expression profiling of murine eGFP+ relfl/flCg1-Cre and eGFP− Cg1-Cre splenic germinal center B cells identifies genes regulated by the transcription factor c-REL in germinal center B cells. Germinal center B cells from 12-week old relfl/flCg1-Cre and Cg1-Cre littermate mice immunized with sheep red blood cells (SRBC) were isolated at day 7 after immunization by flow cytometric sorting from splenic mononuclear cells. RNA was isolated. Following a cDNA amplification step, labeled cRNA was hybridized to microarrays and genes specifically expressed in eGFP+ relfl/flCg1-Cre and eGFP− Cg1-Cre samples were identified by supervised analysis.

2014-07-15 | E-GEOD-58839 | biostudies-arrayexpress

RNA-seq data from murine eGFP+ relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre splenic germinal center B cells purified by fluorescent activated cell sorting.

Project description:RNA-seq analysis of murine eGFP+ relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre splenic germinal center B cells identifies genes regulated by the transcription factors RELB and p52 (NF-kB2) in germinal center B cells. Germinal center B cells from 12-week old relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre littermate mice immunized with sheep red blood cells (SRBC) were isolated at day 7 after immunization by flow cytometric sorting from splenic mononuclear cells. RNA was isolated, amplified and submitted for RNA-sequencing on an Illumina HiSeq2500 instrument for 35-40 million 2x50 paired-ended reads.

2016-07-01 | E-GEOD-77374 | biostudies-arrayexpress

Mus musculus

Project description:Expression data from murine eGFP+ relfl/flCg1-Cre and eGFP-Cg1-Cre splenic germinal center B cells purified by fluorescent activated cell sorting.

| PRJNA253672 | ENA

Expression data from murine eGFP+ relfl/flCg1-Cre and eGFP-Cg1-Cre splenic germinal center B cells purified by fluorescent activated cell sorting.

2014-07-15 | GSE58839 | GEO

RNA-seq data from murine eGFP+ relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre splenic germinal center B cells purified by fluorescent activated cell sorting.

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Project description:The chemokines CXCL13 and CXCL12 are reported to be important for the germinal center reaction. Since CXCL12-deficient mice are embryonically lethal, here we took advantage of the Cxcl13-Cre/TdTomato mouse models to genetically ablate CXCL12 from B cell-interacting reticular cells and examine the molecular consequence on germinal center B cells. Spatial segregation of follicular dendritic cells, germinal center B cells and follicular helper T cells is impaired in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice. Single cell transcriptomic analysis revealed that all germinal center B cell subsets (corresponding to distinct stages of the germinal center response) are present in draining lymph nodes of immunized CXCL12-conditionally deficient mice. While most transcriptional regulators of the germinal center response are unperturbed by the genetic perturbation of CXCL12, Bach2 levels were elevated in germinal center B cells from lymph nodes of Cxcl12fl/fl mice. Moreover, single cell B cell receptor sequencing revealed that germinal center B cells in Cxcl13-Cre/TdTomato Cxcl12fl/fl mice harbour a lower mutational burden when compared to germinal center B cells isolated from immunized control mice. Gene expression profiles were validated by flow cytometry and suggest that the provision of CXCL12 by reticular cells governs efficient germinal center responses.

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